Manganese (II) oxidation by microperoxidase-11 in hydrophilic organic media

Masafumi Oyadomari, Mari Kabuto, Hiroyuki Wariishi, Hiroo Tanaka

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Microperoxidase-11 (MP-11) derived from horse heart cytochrome c was utilized as a peroxidative catalyst. MnII-oxidation activity of MP-11 was demonstrated for the first time. Although the activity of MP-11 was low compared to manganese peroxidase (MnP) from Phanerochaete chrysosporium in aqueous system, it can be utilized in water-miscible organic solvents if at least 5% water exists. MnII-oxidation by MP-11 was optimized at higher pH compared to MnP, probably because of the lack of distal His acting as a proton acceptor during the catalytic cycle. Finally, the depolymerization of lignin was also achieved in 90% methanol using MP-11 in the presence of H2O2 and MnII.

Original languageEnglish
Pages (from-to)159-164
Number of pages6
JournalBiochemical Engineering Journal
Volume15
Issue number3
DOIs
Publication statusPublished - Sep 1 2003

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Environmental Engineering
  • Bioengineering
  • Biomedical Engineering

Fingerprint Dive into the research topics of 'Manganese (II) oxidation by microperoxidase-11 in hydrophilic organic media'. Together they form a unique fingerprint.

Cite this