Mapping and pyramiding of two major genes for resistance to the brown planthopper (Nilaparvata lugens [Stål]) in the rice cultivar ADR52

Khin Khin Marlar Myint, Daisuke Fujita, Masaya Matsumura, Tomohiro Sonoda, Atsushi Yoshimura, Hideshi Yasui

Research output: Contribution to journalArticle

46 Citations (Scopus)

Abstract

The brown planthopper (BPH), Nilaparvata lugens (Stål), is one of the most serious and destructive pests of rice, and can be found throughout the rice-growing areas of Asia. To date, more than 24 major BPH-resistance genes have been reported in several Oryza sativa ssp. indica cultivars and wild relatives. Here, we report the genetic basis of the high level of BPH resistance derived from an Indian rice cultivar, ADR52, which was previously identified as resistant to the whitebacked planthopper (Sogatella furcifera [Horváth]). An F 2 population derived from a cross between ADR52 and a susceptible cultivar, Taichung 65 (T65), was used for quantitative trait locus (QTL) analysis. Antibiosis testing showed that multiple loci controlled the high level of BPH resistance in this F 2 population. Further linkage analysis using backcross populations resulted in the identification of BPH-resistance (antibiosis) gene loci from ADR52. BPH25 co-segregated with marker S00310 on the distal end of the short arm of chromosome 6, and BPH26 co-segregated with marker RM5479 on the long arm of chromosome 12. To characterize the virulence of the most recently migrated BPH strain in Japan, preliminary near-isogenic lines (pre-NILs) and a preliminary pyramided line (pre-PYL) carrying BPH25 and BPH26 were evaluated. Although both pre-NILs were susceptible to the virulent BPH strain, the pre-PYL exhibited a high level of resistance. The pyramiding of resistance genes is therefore likely to be effective for increasing the durability of resistance against the new virulent BPH strain in Japan.

Original languageEnglish
Pages (from-to)495-504
Number of pages10
JournalTheoretical and Applied Genetics
Volume124
Issue number3
DOIs
Publication statusPublished - Jan 1 2012

Fingerprint

Nilaparvata lugens
major genes
Antibiosis
rice
cultivars
Genes
Japan
Population
Sogatella furcifera
Chromosomes, Human, Pair 12
Chromosomes, Human, Pair 6
Quantitative Trait Loci
antibiosis
isogenic lines
Virulence
chromosomes
Oryza
loci
genes
wild relatives

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Agronomy and Crop Science
  • Genetics

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Mapping and pyramiding of two major genes for resistance to the brown planthopper (Nilaparvata lugens [Stål]) in the rice cultivar ADR52. / Myint, Khin Khin Marlar; Fujita, Daisuke; Matsumura, Masaya; Sonoda, Tomohiro; Yoshimura, Atsushi; Yasui, Hideshi.

In: Theoretical and Applied Genetics, Vol. 124, No. 3, 01.01.2012, p. 495-504.

Research output: Contribution to journalArticle

Myint, Khin Khin Marlar ; Fujita, Daisuke ; Matsumura, Masaya ; Sonoda, Tomohiro ; Yoshimura, Atsushi ; Yasui, Hideshi. / Mapping and pyramiding of two major genes for resistance to the brown planthopper (Nilaparvata lugens [Stål]) in the rice cultivar ADR52. In: Theoretical and Applied Genetics. 2012 ; Vol. 124, No. 3. pp. 495-504.
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