Mapping of transregulatory domains of pseudorabies virus early protein 0 and identification of its dominant-negative mutant

S. Watanabe; Etsuro Ono; Y. Shimizu; H. Kida

doi:10.1007/BF01718604

Mapping of transregulatory domains of pseudorabies virus early protein 0 and identification of its dominant-negative mutant

S. Watanabe, Etsuro Ono, Y. Shimizu, H. Kida

Research output: Contribution to journal › Article

13 Citations (Scopus)

Abstract

Pseudorabies virus (PRV) early protein 0 (EP0) is a transactivator containing the RING finger domain. Analysis of transactivating activity of truncated forms of the EP0 molecule consisting of 410 amino acids revealed that amino-terminal region containing the RING finger domain, amino acids 1 to 84, and the region between amino acids 114 to 242 containing acidic amino acid sequences were required for the transactivation. On the other hand, the mutant consisting of amino acids 1 to 113 exhibited a dominant- negative property.

Original language	English
Pages (from-to)	1001-1009
Number of pages	9
Journal	Archives of Virology
Volume	141
Issue number	6
DOIs	https://doi.org/10.1007/BF01718604
Publication status	Published - Jan 1 1996
Externally published	Yes

Fingerprint

Suid Herpesvirus 1

RING Finger Domains

Amino Acids

Proteins

Acidic Amino Acids

Trans-Activators

Transcriptional Activation

Amino Acid Sequence

All Science Journal Classification (ASJC) codes

Virology

Cite this

Watanabe, S., Ono, E., Shimizu, Y., & Kida, H. (1996). Mapping of transregulatory domains of pseudorabies virus early protein 0 and identification of its dominant-negative mutant. Archives of Virology, 141(6), 1001-1009. https://doi.org/10.1007/BF01718604

Mapping of transregulatory domains of pseudorabies virus early protein 0 and identification of its dominant-negative mutant. / Watanabe, S.; Ono, Etsuro; Shimizu, Y.; Kida, H.

In: Archives of Virology, Vol. 141, No. 6, 01.01.1996, p. 1001-1009.

Research output: Contribution to journal › Article

Watanabe, S, Ono, E, Shimizu, Y & Kida, H 1996, 'Mapping of transregulatory domains of pseudorabies virus early protein 0 and identification of its dominant-negative mutant', Archives of Virology, vol. 141, no. 6, pp. 1001-1009. https://doi.org/10.1007/BF01718604

Watanabe S, Ono E, Shimizu Y, Kida H. Mapping of transregulatory domains of pseudorabies virus early protein 0 and identification of its dominant-negative mutant. Archives of Virology. 1996 Jan 1;141(6):1001-1009. https://doi.org/10.1007/BF01718604

Watanabe, S. ; Ono, Etsuro ; Shimizu, Y. ; Kida, H. / Mapping of transregulatory domains of pseudorabies virus early protein 0 and identification of its dominant-negative mutant. In: Archives of Virology. 1996 ; Vol. 141, No. 6. pp. 1001-1009.

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10.1007/BF01718604