Mass Fragmentographic Determination of γ‐Aminobutyric Acid and Glutamic Acid in Discrete Amygdaloid Nuclei of Rat Brain

Kaoru Nakamura, Takashi Matsumoto, Makoto Hirano, Hiroshi Kagoshima, Toshihide Kuroki, Hiroshi Yao, Hideyuki Uchimura, Tatsuo Nakahara

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6 Citations (Scopus)


Abstract: A mass fragmentographic method for the simultaneous quantification of γ‐aminobutyric acid (GABA) and glutamic acid is described. In a convenient one‐step reaction, the two ammo acids were derivatized with pentafluor‐opropionic anhydride and pentafluoropropanol. The deri‐vatization products were stable for several days. The technique has been applied to the assay of GABA and Glu in five amygdaloid nuclei of the rat brain. The GABA level was high in the central and medial nuclei, whereas the Glu level was high in the lateral and basal nuclei. The regional distribution of GABA was different from that of Glu within the amygdaloid nuclei.

Original languageEnglish
Pages (from-to)1842-1844
Number of pages3
JournalJournal of Neurochemistry
Issue number6
Publication statusPublished - Jun 1987
Externally publishedYes


All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Cellular and Molecular Neuroscience

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