Mass spectrometry-based approaches toward absolute quantitative proteomics

Keiji Kito, Takashi Ito

Research output: Contribution to journalReview article

69 Citations (Scopus)

Abstract

Mass spectrometry has served as a major tool for the discipline of proteomics to catalogue proteins in an unprecedented scale. With chemical and metabolic techniques for stable isotope labeling developed over the past decade, it is now routinely used as a method for relative quantification to provide valuable information on alteration of protein abundance in a proteome-wide scale. More recently, absolute or stoichiometric quantification of proteome is becoming feasible, in particular, with the development of strategies with isotope-labeled standards composed of concatenated peptides. On the other hand, remarkable progress has been also made in label-free quantification methods based on the number of identified peptides, Here we review these mass spectrometry-based approaches for absolute quantification of proteome and discuss their implications.

Original languageEnglish
Pages (from-to)263-274
Number of pages12
JournalCurrent Genomics
Volume9
Issue number4
DOIs
Publication statusPublished - Jun 1 2008

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Proteome
Proteomics
Mass Spectrometry
Isotope Labeling
Peptides
Isotopes
Proteins

All Science Journal Classification (ASJC) codes

  • Genetics
  • Genetics(clinical)

Cite this

Mass spectrometry-based approaches toward absolute quantitative proteomics. / Kito, Keiji; Ito, Takashi.

In: Current Genomics, Vol. 9, No. 4, 01.06.2008, p. 263-274.

Research output: Contribution to journalReview article

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