Mass-tag technology for monitoring of protein kinase activity using mass spectrometry

Tatsuhiko Sonoda, Syuhei Shigaki, Takeyuki Nagashima, Osamu Okitsu, Yasuhiro Kita, Masaharu Murata, Yoshiki Katayama

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

Monitoring of intracellular protein kinase activity is very important for fields involving diagnosis and drug screening. However, current methods, such as radiometry using 32P, or ELISA, are laborious and time-consuming. We have developed high-throughput assay system of protein kinase activity using mass-tagged substrate peptide probes and mass spectrometry. This assay system can easily evaluate target kinase activity and will potentially be able to simultaneously profile many protein kinase activities.

Original languageEnglish
Pages (from-to)847-850
Number of pages4
JournalBioorganic and Medicinal Chemistry Letters
Volume14
Issue number4
DOIs
Publication statusPublished - Feb 23 2004

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Molecular Medicine
  • Molecular Biology
  • Pharmaceutical Science
  • Drug Discovery
  • Clinical Biochemistry
  • Organic Chemistry

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