Massive gene transfer and extensive RNA editing of a symbiotic dinoflagellate plastid genome

Sutada Mungpakdee, Chuya Shinzato, Takeshi Takeuchi, Takeshi Kawashima, Ryo Koyanagi, Kanako Hisata, Makiko Tanaka, Hiroki Goto, Manabu Fujie, Senjie Lin, Nori Satoh, Eiichi Shoguchi

    Research output: Contribution to journalArticle

    38 Citations (Scopus)

    Abstract

    Genome sequencing of Symbiodinium minutum revealed that 95 of 109 plastid-associated genes have been transferred to the nucleargenomeandsubsequently expandedbygeneduplication.Only14genes remain inplastidsandoccur asDNAminicircles. Each minicircle (1.8-3.3 kb) contains one gene and a conserved noncoding region containing putative promoters and RNA-binding sites. Nine types of RNA editing, including a novel G/U type, were discovered in minicircle transcripts but not in genes transferred to the nucleus. In contrast toDNAediting sites in dinoflagellatemitochondria,which tend to be highly conserved across all taxa, editing sites employed in DNA minicircles are highly variable from species to species. Editing is crucial for core photosystem protein function. It restores evolutionarily conserved aminoacids andincreasespeptidyl hydropathy. It alsoincreases proteinplasticitynecessary to initiate photosystem complex assembly.

    Original languageEnglish
    Pages (from-to)1408-1422
    Number of pages15
    JournalGenome Biology and Evolution
    Volume6
    Issue number6
    DOIs
    Publication statusPublished - Jun 2014

    Fingerprint

    Plastid Genomes
    RNA Editing
    RNA editing
    Dinoflagellida
    gene transfer
    plastid
    Transfer RNA
    dinoflagellate
    RNA
    genome
    gene
    Genes
    Symbiodinium
    Plastids
    genes
    plastids
    binding sites
    Binding Sites
    promoter regions
    Genome

    All Science Journal Classification (ASJC) codes

    • Ecology, Evolution, Behavior and Systematics
    • Genetics

    Cite this

    Mungpakdee, S., Shinzato, C., Takeuchi, T., Kawashima, T., Koyanagi, R., Hisata, K., ... Shoguchi, E. (2014). Massive gene transfer and extensive RNA editing of a symbiotic dinoflagellate plastid genome. Genome Biology and Evolution, 6(6), 1408-1422. https://doi.org/10.1093/gbe/evu109

    Massive gene transfer and extensive RNA editing of a symbiotic dinoflagellate plastid genome. / Mungpakdee, Sutada; Shinzato, Chuya; Takeuchi, Takeshi; Kawashima, Takeshi; Koyanagi, Ryo; Hisata, Kanako; Tanaka, Makiko; Goto, Hiroki; Fujie, Manabu; Lin, Senjie; Satoh, Nori; Shoguchi, Eiichi.

    In: Genome Biology and Evolution, Vol. 6, No. 6, 06.2014, p. 1408-1422.

    Research output: Contribution to journalArticle

    Mungpakdee, S, Shinzato, C, Takeuchi, T, Kawashima, T, Koyanagi, R, Hisata, K, Tanaka, M, Goto, H, Fujie, M, Lin, S, Satoh, N & Shoguchi, E 2014, 'Massive gene transfer and extensive RNA editing of a symbiotic dinoflagellate plastid genome', Genome Biology and Evolution, vol. 6, no. 6, pp. 1408-1422. https://doi.org/10.1093/gbe/evu109
    Mungpakdee S, Shinzato C, Takeuchi T, Kawashima T, Koyanagi R, Hisata K et al. Massive gene transfer and extensive RNA editing of a symbiotic dinoflagellate plastid genome. Genome Biology and Evolution. 2014 Jun;6(6):1408-1422. https://doi.org/10.1093/gbe/evu109
    Mungpakdee, Sutada ; Shinzato, Chuya ; Takeuchi, Takeshi ; Kawashima, Takeshi ; Koyanagi, Ryo ; Hisata, Kanako ; Tanaka, Makiko ; Goto, Hiroki ; Fujie, Manabu ; Lin, Senjie ; Satoh, Nori ; Shoguchi, Eiichi. / Massive gene transfer and extensive RNA editing of a symbiotic dinoflagellate plastid genome. In: Genome Biology and Evolution. 2014 ; Vol. 6, No. 6. pp. 1408-1422.
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