Material-Driven Fibronectin Assembly Promotes Maintenance of Mesenchymal Stem Cell Phenotypes

Patricia Rico, Hayk Mnatsakanyan, Matthew J. Dalby, Manuel Salmerón-Sánchez

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

Mesenchymal stem cells (MSCs) are a research tool to investigate fundamental biology and are candidates for use in regenerative medicine. In this context, significant efforts have been devoted to develop technologies to control stem cell fate, including the use of soluble factors in media. However, material properties offer alternative approaches that avoid the use of soluble factors. Here, a material system capable of sustaining the growth of stem cells (maintaining stemness) and of promoting highly efficient differentiation upon external stimulation is described. Poly(ethyl acrylate) induces assembly of fibronectin (FN) into nanonetworks (FN fibrillogenesis) upon simple adsorption from solutions. It is shown that these FN nanonetworks allow growth of MSCs and maintenance of stemness for long periods of time (up to 30 d) using basal media in absence of soluble factors. Additionally, the system promotes enhanced levels of differentiation when defined supplemented media are used. The study reveals the critical role of the intermediate protein layer at the material interface to control MSC fate regardless of the properties of the underlying material and it introduces a new material system as a candidate to be used in MSC niche design.

Original languageEnglish
Pages (from-to)6563-6573
Number of pages11
JournalAdvanced Functional Materials
Volume26
Issue number36
DOIs
Publication statusPublished - Sep 26 2016

All Science Journal Classification (ASJC) codes

  • Chemistry(all)
  • Materials Science(all)
  • Condensed Matter Physics

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