Maternal exposure to dioxin reduces hypothalamic but not pituitary metabolome in fetal rats: A possible mechanism for a fetus-specific reduction in steroidogenesis

Yuki Matsumoto, Takumi Ishida, Tomoki Takeda, Takayuki Koga, Misaki Fujii, Yuji Ishii, Yoshinori Fujimura, Daisuke Miura, Hiroyuki Wariishi, Hideyuki Yamada

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

2,3,7,8-Tetrachlorodibenzo-p-dioxin (TCDD) reduces the synthesis of pituitary gonadotropins in a fetal age-specific manner. The pituitary synthesis of gonadotropins is regulated by the hypothalamus and, thus, needs the differentiation and development of the hypothalamus requiring a number of factors including energy supply and neurotransmitters. To investigate the mechanism whereby TCDD reduces fetal gonadotropins, we carried out a comparative study on the metabolomes of the hypothalamus and pituitary using fetal and mature Wistar rats. Male fetuses at gestational day (GD)20 were removed from dams treated orally with TCDD (1 μg/kg) at GD15, and the metabolome profiles were analyzed by gas chromatography-mass spectrometry (GC-MS). The principal component analysis of GC-MS data revealed that TCDD caused a change in the profile of fetal metabolome more markedly in the hypothalamus than in the pituitary. In sharp contrast, TCDD did not cause any marked alteration in hypothalamic as well as pituitary metabolomes in male rats born of untreated dams and treated with TCDD at postnatal day 49. It was also demonstrated that a number of fetal hypothalamic components, including glutamine and γ-aminobutyric acid, are reduced by TCDD. These results demonstrate a possibility that TCDD may reduce the metabolic activity of the hypothalamus in a fetus-specific fashion, resulting in the reduced synthesis of gonadotropins.

Original languageEnglish
Pages (from-to)365-373
Number of pages9
JournalJournal of Toxicological Sciences
Volume35
Issue number3
DOIs
Publication statusPublished - Jan 1 2010

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Maternal Exposure
Dioxins
Metabolome
Rats
Fetus
Hypothalamus
Gonadotropins
Pituitary Gonadotropins
Gas chromatography
Gas Chromatography-Mass Spectrometry
Dams
Mass spectrometry
Aminobutyrates
Polychlorinated Dibenzodioxins
1,4-dioxin
Principal Component Analysis
Glutamine
Principal component analysis
Gestational Age
Neurotransmitter Agents

All Science Journal Classification (ASJC) codes

  • Toxicology

Cite this

Maternal exposure to dioxin reduces hypothalamic but not pituitary metabolome in fetal rats : A possible mechanism for a fetus-specific reduction in steroidogenesis. / Matsumoto, Yuki; Ishida, Takumi; Takeda, Tomoki; Koga, Takayuki; Fujii, Misaki; Ishii, Yuji; Fujimura, Yoshinori; Miura, Daisuke; Wariishi, Hiroyuki; Yamada, Hideyuki.

In: Journal of Toxicological Sciences, Vol. 35, No. 3, 01.01.2010, p. 365-373.

Research output: Contribution to journalArticle

Matsumoto, Yuki ; Ishida, Takumi ; Takeda, Tomoki ; Koga, Takayuki ; Fujii, Misaki ; Ishii, Yuji ; Fujimura, Yoshinori ; Miura, Daisuke ; Wariishi, Hiroyuki ; Yamada, Hideyuki. / Maternal exposure to dioxin reduces hypothalamic but not pituitary metabolome in fetal rats : A possible mechanism for a fetus-specific reduction in steroidogenesis. In: Journal of Toxicological Sciences. 2010 ; Vol. 35, No. 3. pp. 365-373.
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