Maternal protein restriction alters the renal ptger1 DNA methylation state in SHRSP offspring

Moe Miyoshi, Masayuki Sato, Kenji Saito, Lila Otani, Katsuhiko Shirahige, Fumihito Miura, Takashi Ito, Huijuan Jia, Hisanori Kato

Research output: Contribution to journalArticle

Abstract

We previously reported that maternal protein restriction (LP) during pregnancy increases salt sensitivity in offspring using the Stroke-Prone Spontaneously Hypertensive Rat (SHRSP). In the present study, we focus on DNA methylation profiles of prostaglandin E receptor 1 gene (ptger1), which is known to be associated with hypertension. We evaluated the ptger1 DNA methylation status via bisulfite sequencing, and analyzed the expression of ptger1-related genes. The results of these analyses showed that, compared to controls, the LP-S offspring exhibited both marked ptger1 hypermethylation, and significantly increased ptger1 expression. Moreover, they also exhibited significantly decreased expression of the downstream gene epithelial Na+ channel alpha (enacα). Interestingly, LP offspring that were provided with a standard water drinking supply (W) also exhibited increased ptger1 methylation and expression. Together, these results suggest that maternal protein restriction during pregnancy modulates the renal ptger1 DNA methylation state in SHRSP offspring, and thereby likely mediates ptger1 and enacα gene expression to induce salt sensitivity.

Original languageEnglish
Article number1436
JournalNutrients
Volume10
Issue number10
DOIs
Publication statusPublished - Oct 5 2018

All Science Journal Classification (ASJC) codes

  • Food Science
  • Nutrition and Dietetics

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    Miyoshi, M., Sato, M., Saito, K., Otani, L., Shirahige, K., Miura, F., Ito, T., Jia, H., & Kato, H. (2018). Maternal protein restriction alters the renal ptger1 DNA methylation state in SHRSP offspring. Nutrients, 10(10), [1436]. https://doi.org/10.3390/nu10101436