Maturation-inducing hormone of the tiger puffer, Takifugu rubripes (tetraodontidae, teleostei): Biosynthesis of steroids by the ovaries and the relative effectiveness of steroid metabolites for germinal vesicle breakdown in vitro

Michiya Matsuyama, Ayane Sasaki, Koh Nakagawa, Tohru Kobayashi, Yoshitaka Nagahama, Hisashi Chuda

Research output: Contribution to journalArticlepeer-review

17 Citations (Scopus)

Abstract

Yolk-free follicle cells during final meiotic maturation were incubated with a radioactively labeled steroid precursor, and the maturation-inducing activity of the steroid metabolites produced was tested in an in vitro assay based on GVBD. Of the metabolites obtained by TLC, whose purity and final characterization were confirmed by recrystallization to constant specific activity, two steroids, 17,20β-P and 20β-S, exhibited maturation-inducing activity in vitro. However, 20β-S was overwhelmingly more effective and faster at inducing GVBD in vitro than 17,20β-P. During final oocyte maturation in the tiger puffer, a shift in steroidogenic enzymes, a decrease in C17,20-lyase activity, and an increase in 20ß-HSD activity, were found, as previously reported in salmonids and medaka. In addition to this shift in steroidogenic enzyme activity, however, high and increasing 21-hydroxylase activity throughout all the oocyte developmental stages was a distinctive feature in tiger puffer follicles. This 21-hydroxylase activity likely enables ovarian follicles to accumulate enough 11-deoxycortisol during vitellogenesis for 20β-S production on a massive scale during GVBD. Thus, this study provides not only evidence on the physiological role of 20β-S as a MIH in the tiger puffer, but also fact on enzymatic kinetics in 20β-S biosynthesis.

Original languageEnglish
Pages (from-to)225-234
Number of pages10
JournalZoological science
Volume18
Issue number2
DOIs
Publication statusPublished - Mar 1 2001

All Science Journal Classification (ASJC) codes

  • Animal Science and Zoology

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