Maturation-promoting factor and p34cdc2 kinase during oocyte maturation of the Japanese quail

M. Mori, M. Yamashita, M. Yoshikuni, S. Fukada, Y. Nagahama

Research output: Contribution to journalArticlepeer-review

11 Citations (Scopus)

Abstract

Maturation-promoting factor and a homolog of fission yeast cdc2+ gene product (p34cdc2) were investigated during the final 24 hr of maturation of quail oocytes. Kinase activity of p34cdc2 in the oocyte germinal disk (GD) increased 15 times at maturation. Two bands, at 32 and 34 kDa, were detected in immature oocytes by immunoblotting of SDS-PAGE with anti-p34cdc2 monoclonal antibody. A new band, which is close to the 32-kDa band but with a slightly faster mobility, appeared during maturation. No p34cdc2 could be detected outside the GD. Microinjection of GD extract from mature oocytes caused maturation of Xenopus oocytes.

Original languageEnglish
Pages (from-to)246-249
Number of pages4
JournalDevelopmental Biology
Volume146
Issue number1
DOIs
Publication statusPublished - Jul 1991
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Molecular Biology
  • Developmental Biology
  • Cell Biology

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