MCP-1 and RANTES are expressed in renal cortex of rats chronically treated with nitric oxide synthase inhibitor: Involvement in macrophage and monocyte recruitment

Minoru Kashiwagi, Kohsuke Masutani, Michiya Shinozaki, Hideki Hirakata

Research output: Contribution to journalArticle

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Abstract

Long-term inhibition of nitric oxide synthase (NOS) in rats is known to cause systemic hypertension and renal parenchymal injury. We have previously reported that activation of intra-renal renin-angiotensin system was a major contributing factor for renal injury in chronically NOS-inhibited rats. Massive interstitial infiltration of monocytes/macrophages (M/M) was characteristically seen in this model. The present study was performed to elucidate the role of chemokines, RANTES and MCP-1, in promoting M/M recruitment into the renal cortex. The number of infiltrating ED-1-positive cells was examined in association with the level of expression of RANTES and MCP1 mRNAs in the renal cortex of rats treated orally for 12 weeks with L-NAME. Compared to controls rats, the number of infiltrating ED-1-positive cells was significantly higher in L-NAME-treated rats. The mRNA expressions of both RANTES and MCP-1 were significantly higher in L-NAME-treated rats than the control. In L-NAME-treated rats, the high number of ED-1-positive cells and increased expression of both RANTES and MCP-1 were suppressed by ACE inhibitor, but not by hydralazine. In contrast, neither ED-1 counts nor RANTES mRNA expression were affected by angiotensin (Ang) II type 1 receptor antagonist. These results suggest the likely involvement of RANTES and MCP-1 in the recruitment of M/M into the renal cortex of rats with chronic NOS inhibition. Furthermore, it is also indicated that Ang II stimulates MCP-1 expression via Ang II type 1 receptor, whereas RANTES expression is mediated via Ang II type 2 receptor.

Original languageEnglish
Pages (from-to)165-173
Number of pages9
JournalNephron
Volume92
Issue number1
DOIs
Publication statusPublished - 2002

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Chemokine CCL5
Nitric Oxide Synthase
Monocytes
Macrophages
Kidney
NG-Nitroarginine Methyl Ester
Messenger RNA
Angiotensin Type 2 Receptor
Angiotensin II Type 1 Receptor Blockers
Hydralazine
Angiotensin Type 1 Receptor
Renal Hypertension
Wounds and Injuries
Renin-Angiotensin System
Chemokines
Angiotensin-Converting Enzyme Inhibitors
Angiotensin II

All Science Journal Classification (ASJC) codes

  • Nephrology

Cite this

MCP-1 and RANTES are expressed in renal cortex of rats chronically treated with nitric oxide synthase inhibitor : Involvement in macrophage and monocyte recruitment. / Kashiwagi, Minoru; Masutani, Kohsuke; Shinozaki, Michiya; Hirakata, Hideki.

In: Nephron, Vol. 92, No. 1, 2002, p. 165-173.

Research output: Contribution to journalArticle

Kashiwagi, Minoru ; Masutani, Kohsuke ; Shinozaki, Michiya ; Hirakata, Hideki. / MCP-1 and RANTES are expressed in renal cortex of rats chronically treated with nitric oxide synthase inhibitor : Involvement in macrophage and monocyte recruitment. In: Nephron. 2002 ; Vol. 92, No. 1. pp. 165-173.
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