Measles virus circumvents the host interferon response by different actions of the C and V proteins

Yuichiro Nakatsu, Makoto Takeda, Shinji Ohno, Yuta Shirogane, Masaharu Iwasaki, Yusuke Yanagi

Research output: Contribution to journalArticle

75 Citations (Scopus)

Abstract

Measles is an acute febrile infectious disease with high morbidity and mortality. The genome of measles virus (MV), the causative agent, encodes two accessory products, V and C proteins, that play important roles in MV virulence. The V but not the C protein of the IC-B strain (a well-characterized virulent strain of MV) has been shown to block the Jak/Stat signaling pathway and counteract the cellular interferon (IFN) response. We have recently shown that a recombinant IC-B strain that lacks C protein expression replicates poorly in certain cell lines, and its growth defect is related to translational inhibition and strong IFN induction. Here, we show that the V protein of the MV IC-B strain also blocks the IFN induction pathway mediated by the melanoma differentiation-associated gene 5 product, thus actively interfering with the host IFN response at two different steps. On the other hand, the C protein per se possesses no activity to block the IFN induction pathway. Our data indicate that the C protein acts as a regulator of viral RNA synthesis, thereby acting indirectly to suppress IFN induction. Since recombinant MVs with C protein defective in modulating viral RNA synthesis or lacking C protein expression strongly stimulate IFN production, in spite of V protein production, both the C and V proteins must be required for MV to fully circumvent the host IFN response.

Original languageEnglish
Pages (from-to)8296-8306
Number of pages11
JournalJournal of virology
Volume82
Issue number17
DOIs
Publication statusPublished - Sep 1 2008

Fingerprint

Measles virus
interferons
Protein C
Interferons
proteins
Viral RNA
protein synthesis
RNA
synthesis
Measles
melanoma
infectious diseases
fever
Communicable Diseases
morbidity
Virulence
Melanoma
Fever
virulence
cell lines

All Science Journal Classification (ASJC) codes

  • Microbiology
  • Immunology
  • Insect Science
  • Virology

Cite this

Measles virus circumvents the host interferon response by different actions of the C and V proteins. / Nakatsu, Yuichiro; Takeda, Makoto; Ohno, Shinji; Shirogane, Yuta; Iwasaki, Masaharu; Yanagi, Yusuke.

In: Journal of virology, Vol. 82, No. 17, 01.09.2008, p. 8296-8306.

Research output: Contribution to journalArticle

Nakatsu, Yuichiro ; Takeda, Makoto ; Ohno, Shinji ; Shirogane, Yuta ; Iwasaki, Masaharu ; Yanagi, Yusuke. / Measles virus circumvents the host interferon response by different actions of the C and V proteins. In: Journal of virology. 2008 ; Vol. 82, No. 17. pp. 8296-8306.
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