Measles virus infects both polarized epithelial and immune cells by using distinctive receptor-binding sites on its hemagglutinin

Maino Tahara, Makoto Takeda, Yuta Shirogane, Takao Hashiguchi, Shinji Ohno, Yusuke Yanagi

Research output: Contribution to journalArticle

85 Citations (Scopus)

Abstract

Measles is one of the most contagious human infectious diseases and remains a major cause of childhood morbidity and mortality worldwide. The signaling lymphocyte activation molecule (SLAM), also called CD150, is a cellular receptor for measles virus (MV), presumably accounting for its tropism for immune cells and its immunosuppressive properties. On the other hand, pathological studies have shown that MV also infects epithelial cells at a later stage of infection, although its mechanism has so far been unknown. In this study, we show that wild-type MV can infect and produce syncytia in human polarized epithelial cell lines independently of SLAM and CD46 (a receptor for the vaccine strains of MV). Progeny viral particles are released exclusively from the apical surface of these polarized epithelial cell lines. We have also identified amino acid residues on the MV attachment protein that are likely to interact with a putative receptor on epithelial cells. All of these residues have aromatic side chains and may form a receptor-binding pocket located in a different position from the putative SLAM- and CD46-binding sites on the MV attachment protein. Thus, our results indicate that MV has an intrinsic ability to infect both polarized epithelial and immune cells by using distinctive receptor-binding sites on the attachment protein corresponding to each of their respective receptors. The ability of MV to infect polarized epithelial cells and its exclusive release from the apical surface may facilitate its efficient transmission via aerosol droplets, resulting in its highly contagious nature.

Original languageEnglish
Pages (from-to)4630-4637
Number of pages8
JournalJournal of virology
Volume82
Issue number9
DOIs
Publication statusPublished - May 1 2008

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Measles virus
Hemagglutinins
hemagglutinins
binding sites
Epithelial Cells
Binding Sites
receptors
epithelial cells
lymphocyte proliferation
Lymphocyte Activation
cells
Virus Attachment
cell lines
immunosuppressive agents
Cell Line
tropisms
Proteins
Tropism
proteins
Measles

All Science Journal Classification (ASJC) codes

  • Microbiology
  • Immunology
  • Insect Science
  • Virology

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Measles virus infects both polarized epithelial and immune cells by using distinctive receptor-binding sites on its hemagglutinin. / Tahara, Maino; Takeda, Makoto; Shirogane, Yuta; Hashiguchi, Takao; Ohno, Shinji; Yanagi, Yusuke.

In: Journal of virology, Vol. 82, No. 9, 01.05.2008, p. 4630-4637.

Research output: Contribution to journalArticle

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