Measles virus nonstructural C protein modulates viral RNA polymerase activity by interacting with host protein SHCBP1

Minako Ito, Masaharu Iwasaki, Makoto Takeda, Takanori Nakamura, Yusuke Yanagi, Shinji Ohno

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

Most viruses possess strategies to circumvent host immune responses. The measles virus (MV) nonstructural C protein suppresses the interferon response, thereby allowing efficient viral growth, but its detailed mechanism has been unknown. We identified Shc Src homology 2 domain-binding protein 1 (SHCBP1) as one of the host proteins interacting with the C protein. Knockdown of SHCBP1 using a short-hairpin RNA greatly reduced MV growth. SHCBP1 was found to be required for viral RNA synthesis in the minigenome assay and to bind to the MV phosphoprotein, a subunit of the viral RNA polymerase. A stretch of 12 amino acid residues in the C protein were sufficient for SHCBP1 binding, and the peptide containing these 12 residues could suppress MV RNA synthesis, like the full-length C protein. The central region of SHCBP1 was found to bind to the C protein, as well as the phosphoprotein, but the two viral proteins did not compete for SHCBP1 binding. Our results indicate that the C protein modulates MV RNA polymerase activity by binding to the host protein SHCBP1. SHCBP1 may be exploited as a target of antiviral compounds.

Original languageEnglish
Pages (from-to)9633-9642
Number of pages10
JournalJournal of virology
Volume87
Issue number17
DOIs
Publication statusPublished - Sep 5 2013

Fingerprint

Measles virus
viral proteins
Viral RNA
DNA-Directed RNA Polymerases
DNA-directed RNA polymerase
Protein C
Phosphoproteins
Proteins
proteins
phosphoproteins
src Homology Domains
Viral Proteins
Growth
Interferons
Small Interfering RNA
Antiviral Agents
Carrier Proteins
RNA
Viruses
Amino Acids

All Science Journal Classification (ASJC) codes

  • Microbiology
  • Immunology
  • Insect Science
  • Virology

Cite this

Measles virus nonstructural C protein modulates viral RNA polymerase activity by interacting with host protein SHCBP1. / Ito, Minako; Iwasaki, Masaharu; Takeda, Makoto; Nakamura, Takanori; Yanagi, Yusuke; Ohno, Shinji.

In: Journal of virology, Vol. 87, No. 17, 05.09.2013, p. 9633-9642.

Research output: Contribution to journalArticle

Ito, Minako ; Iwasaki, Masaharu ; Takeda, Makoto ; Nakamura, Takanori ; Yanagi, Yusuke ; Ohno, Shinji. / Measles virus nonstructural C protein modulates viral RNA polymerase activity by interacting with host protein SHCBP1. In: Journal of virology. 2013 ; Vol. 87, No. 17. pp. 9633-9642.
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