Abstract
Live attenuated vaccines against measles have been developed through adaptation of clinical isolates of measles virus (MV) in various cultured cells. Analyses using recombinant MVs with chimeric genomes between wild-type and Edmonston vaccine strains indicated that viruses possessing the polymerase protein genes of the Edmonston strain exhibited attenuated viral gene expression and growth in cultured cells as well as in mice expressing an MV receptor, signaling lymphocyte activation molecule, regardless of whether the virus genome had the wild-type or vaccine-type promoter sequence. These data demonstrate that the polymerase protein genes of the Edmonston strain contribute to its attenuated phenotype.
| Original language | English |
|---|---|
| Pages (from-to) | 11979-11984 |
| Number of pages | 6 |
| Journal | Journal of virology |
| Volume | 82 |
| Issue number | 23 |
| DOIs | |
| Publication status | Published - Dec 1 2008 |
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All Science Journal Classification (ASJC) codes
- Microbiology
- Immunology
- Insect Science
- Virology
Cite this
Measles viruses possessing the polymerase protein genes of the Edmonston vaccine strain exhibit attenuated gene expression and growth in cultured cells and SLAM knock-in mice. / Takeda, Makoto; Ohno, Shinji; Tahara, Maino; Takeuchi, Hiroki; Shirogane, Yuta; Ohmura, Hirofumi; Nakamura, Takafumi; Yanagi, Yusuke.
In: Journal of virology, Vol. 82, No. 23, 01.12.2008, p. 11979-11984.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Measles viruses possessing the polymerase protein genes of the Edmonston vaccine strain exhibit attenuated gene expression and growth in cultured cells and SLAM knock-in mice
AU - Takeda, Makoto
AU - Ohno, Shinji
AU - Tahara, Maino
AU - Takeuchi, Hiroki
AU - Shirogane, Yuta
AU - Ohmura, Hirofumi
AU - Nakamura, Takafumi
AU - Yanagi, Yusuke
PY - 2008/12/1
Y1 - 2008/12/1
N2 - Live attenuated vaccines against measles have been developed through adaptation of clinical isolates of measles virus (MV) in various cultured cells. Analyses using recombinant MVs with chimeric genomes between wild-type and Edmonston vaccine strains indicated that viruses possessing the polymerase protein genes of the Edmonston strain exhibited attenuated viral gene expression and growth in cultured cells as well as in mice expressing an MV receptor, signaling lymphocyte activation molecule, regardless of whether the virus genome had the wild-type or vaccine-type promoter sequence. These data demonstrate that the polymerase protein genes of the Edmonston strain contribute to its attenuated phenotype.
AB - Live attenuated vaccines against measles have been developed through adaptation of clinical isolates of measles virus (MV) in various cultured cells. Analyses using recombinant MVs with chimeric genomes between wild-type and Edmonston vaccine strains indicated that viruses possessing the polymerase protein genes of the Edmonston strain exhibited attenuated viral gene expression and growth in cultured cells as well as in mice expressing an MV receptor, signaling lymphocyte activation molecule, regardless of whether the virus genome had the wild-type or vaccine-type promoter sequence. These data demonstrate that the polymerase protein genes of the Edmonston strain contribute to its attenuated phenotype.
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UR - http://www.scopus.com/inward/citedby.url?scp=56449085896&partnerID=8YFLogxK
U2 - 10.1128/JVI.00867-08
DO - 10.1128/JVI.00867-08
M3 - Article
VL - 82
SP - 11979
EP - 11984
JO - Journal of Virology
JF - Journal of Virology
SN - 0022-538X
IS - 23
ER -