Measles viruses possessing the polymerase protein genes of the Edmonston vaccine strain exhibit attenuated gene expression and growth in cultured cells and SLAM knock-in mice

Makoto Takeda, Shinji Ohno, Maino Tahara, Hiroki Takeuchi, Yuta Shirogane, Hirofumi Ohmura, Takafumi Nakamura, Yusuke Yanagi

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27 Citations (Scopus)

Abstract

Live attenuated vaccines against measles have been developed through adaptation of clinical isolates of measles virus (MV) in various cultured cells. Analyses using recombinant MVs with chimeric genomes between wild-type and Edmonston vaccine strains indicated that viruses possessing the polymerase protein genes of the Edmonston strain exhibited attenuated viral gene expression and growth in cultured cells as well as in mice expressing an MV receptor, signaling lymphocyte activation molecule, regardless of whether the virus genome had the wild-type or vaccine-type promoter sequence. These data demonstrate that the polymerase protein genes of the Edmonston strain contribute to its attenuated phenotype.

Original languageEnglish
Pages (from-to)11979-11984
Number of pages6
JournalJournal of virology
Volume82
Issue number23
DOIs
Publication statusPublished - Dec 1 2008

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All Science Journal Classification (ASJC) codes

  • Microbiology
  • Immunology
  • Insect Science
  • Virology

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