TY - JOUR
T1 - Measurement and correlation of liquid-liquid equilibria and partition coefficients of hydrolytic enzymes for DEX T500 + PEG20000 + water aqueous two-phase system at 20°C
AU - Furuya, Takeshi
AU - Yamada, Sunao
AU - Zhu, Jian
AU - Yamaguchi, Yuji
AU - Iwai, Yoshio
AU - Arai, Yasuhiko
PY - 1996/10/15
Y1 - 1996/10/15
N2 - The liquid-liquid equilibria (LLE) for the dextran T500 (DEX T500) + polyethylene glycol) 20000 (PEG20000) + water aqueous two-phase system were measured at 20°C. Furthermore, the molecular weight distributions of DEX and PEG in the top and bottom phases were measured. The partition coefficients (Kp) of hydrolytic enzymes, α-amylase, β-amylase and glucoamylase were also measured. LLE and Kp were correlated using a modified Flory-Huggins equation developed for aqueous systems. The molecular weight distribution of DEX was expressed by a Γ distribution function. LLE and molecular weight distributions were correlated successfully using a continuous thermodynamic approach. The partition coefficients were also correlated with good agreement.
AB - The liquid-liquid equilibria (LLE) for the dextran T500 (DEX T500) + polyethylene glycol) 20000 (PEG20000) + water aqueous two-phase system were measured at 20°C. Furthermore, the molecular weight distributions of DEX and PEG in the top and bottom phases were measured. The partition coefficients (Kp) of hydrolytic enzymes, α-amylase, β-amylase and glucoamylase were also measured. LLE and Kp were correlated using a modified Flory-Huggins equation developed for aqueous systems. The molecular weight distribution of DEX was expressed by a Γ distribution function. LLE and molecular weight distributions were correlated successfully using a continuous thermodynamic approach. The partition coefficients were also correlated with good agreement.
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U2 - 10.1016/s0378-3812(96)03092-0
DO - 10.1016/s0378-3812(96)03092-0
M3 - Article
AN - SCOPUS:0030260331
VL - 125
SP - 89
EP - 102
JO - Fluid Phase Equilibria
JF - Fluid Phase Equilibria
SN - 0378-3812
IS - 1-2
ER -