Measurement and correlation of liquid-liquid equilibria and partition coefficients of hydrolytic enzymes for DEX T500 + PEG20000 + water aqueous two-phase system at 20°C

Takeshi Furuya, Sunao Yamada, Jian Zhu, Yuji Yamaguchi, Yoshio Iwai, Yasuhiko Arai

Research output: Contribution to journalArticlepeer-review

25 Citations (Scopus)

Abstract

The liquid-liquid equilibria (LLE) for the dextran T500 (DEX T500) + polyethylene glycol) 20000 (PEG20000) + water aqueous two-phase system were measured at 20°C. Furthermore, the molecular weight distributions of DEX and PEG in the top and bottom phases were measured. The partition coefficients (Kp) of hydrolytic enzymes, α-amylase, β-amylase and glucoamylase were also measured. LLE and Kp were correlated using a modified Flory-Huggins equation developed for aqueous systems. The molecular weight distribution of DEX was expressed by a Γ distribution function. LLE and molecular weight distributions were correlated successfully using a continuous thermodynamic approach. The partition coefficients were also correlated with good agreement.

Original languageEnglish
Pages (from-to)89-102
Number of pages14
JournalFluid Phase Equilibria
Volume125
Issue number1-2
DOIs
Publication statusPublished - Oct 15 1996

All Science Journal Classification (ASJC) codes

  • Chemical Engineering(all)
  • Physics and Astronomy(all)
  • Physical and Theoretical Chemistry

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