Measurement and correlation of phase equilibria if aqueous two-phase systems and partition coefficients by enzymes

Takeshi Furuya, Katsushige Nakada, Yoshio Iwai, Yasuhiko Arai, Katsuhiko Takeuchi

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

Furuya, T., Nakada, K., Iwai, Y., Arai, Y. and Takeuchi, K., 1990. Measurement and correlation of phase equilibria of aqueous two-phase systems and partition coefficients of enzymes. Aqueous two-phase systems provide a successful method for separating biomolecules by extraction under mild condition. Nevertheless, the phase equilibrium data of aqueous two-phase systems and the partition coefficient data of biomolecules are scarce and it is difficult to correlate them quantitatively. In the present work, the phase equilibria for the dextran-polyethylene glycol-water aqueous two-phase systems are measured. The partition coefficients of enzymes are also measured. Further, the osmotic virial equation is used to correlate the phase equilibria of the aqueous two-phase systems and the partition coefficients of enzymes.

Original languageEnglish
Title of host publicationProcess Metallurgy
Pages1821-1826
Number of pages6
DOIs
Publication statusPublished - Jan 1 1992

Publication series

NameProcess Metallurgy
Volume7
ISSN (Print)1572-4409

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All Science Journal Classification (ASJC) codes

  • Geotechnical Engineering and Engineering Geology
  • Industrial and Manufacturing Engineering

Cite this

Furuya, T., Nakada, K., Iwai, Y., Arai, Y., & Takeuchi, K. (1992). Measurement and correlation of phase equilibria if aqueous two-phase systems and partition coefficients by enzymes. In Process Metallurgy (pp. 1821-1826). (Process Metallurgy; Vol. 7). https://doi.org/10.1016/B978-0-444-88677-4.50119-7