TY - CHAP
T1 - Measurement and correlation of phase equilibria if aqueous two-phase systems and partition coefficients by enzymes
AU - Furuya, Takeshi
AU - Nakada, Katsushige
AU - Iwai, Yoshio
AU - Arai, Yasuhiko
AU - Takeuchi, Katsuhiko
PY - 1992/1/1
Y1 - 1992/1/1
N2 - Furuya, T., Nakada, K., Iwai, Y., Arai, Y. and Takeuchi, K., 1990. Measurement and correlation of phase equilibria of aqueous two-phase systems and partition coefficients of enzymes. Aqueous two-phase systems provide a successful method for separating biomolecules by extraction under mild condition. Nevertheless, the phase equilibrium data of aqueous two-phase systems and the partition coefficient data of biomolecules are scarce and it is difficult to correlate them quantitatively. In the present work, the phase equilibria for the dextran-polyethylene glycol-water aqueous two-phase systems are measured. The partition coefficients of enzymes are also measured. Further, the osmotic virial equation is used to correlate the phase equilibria of the aqueous two-phase systems and the partition coefficients of enzymes.
AB - Furuya, T., Nakada, K., Iwai, Y., Arai, Y. and Takeuchi, K., 1990. Measurement and correlation of phase equilibria of aqueous two-phase systems and partition coefficients of enzymes. Aqueous two-phase systems provide a successful method for separating biomolecules by extraction under mild condition. Nevertheless, the phase equilibrium data of aqueous two-phase systems and the partition coefficient data of biomolecules are scarce and it is difficult to correlate them quantitatively. In the present work, the phase equilibria for the dextran-polyethylene glycol-water aqueous two-phase systems are measured. The partition coefficients of enzymes are also measured. Further, the osmotic virial equation is used to correlate the phase equilibria of the aqueous two-phase systems and the partition coefficients of enzymes.
UR - http://www.scopus.com/inward/record.url?scp=85023466473&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85023466473&partnerID=8YFLogxK
U2 - 10.1016/B978-0-444-88677-4.50119-7
DO - 10.1016/B978-0-444-88677-4.50119-7
M3 - Chapter
AN - SCOPUS:85023466473
T3 - Process Metallurgy
SP - 1821
EP - 1826
BT - Process Metallurgy
ER -