Measurement method for hydroxylated polychlorinated biphenyls in the blood of Yusho patients by liquid chromatography-electrospray tandem mass spectrometry.

Kazuhiro Tobiishi, Takashi Todaka, Hironori Hirakawa, Tsuguhide Hori, Jumboku Kajiwara, Teruaki Hirata, Takao Iida, Uchi Hiroshi, Masutaka Furue

Research output: Contribution to journalArticle

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Abstract

Hydroxylated polychlorinated biphenyls (OH-PCBs) are formed as major metabolites of PCBs by cytochrome P450 enzyme-mediated oxidation. It has been reported that their total concentration in serum samples of Yusho patients ranged from 390 to 1300 pg/g. We developed a measurement method for OH-PCBs in blood samples by LC/MS/MS. This method is effective at determining the concentrations of PCDDs, PCDFs, Co-PCBs and OH-PCBs from the same sample without special treatment of the sample. The concentration of OH-PCBs in the blood of Yusho patients was examined using this method. The major OH-PCB metabolites were 4-OH-CB187 (54-906 pg/g-wet), 4-OH-CB146 + 3-OH-CB153 (32-527 pg/g-wet), 4-OH-CB109 (ND-229 pg/g-wet) and 4'-OH-CB172 (ND-143 pg/g-wet). The total OH-PCBs ranged from 95 to 1740 pg/g-wet.

Original languageEnglish
Pages (from-to)153-158
Number of pages6
JournalFukuoka Acta Medica
Volume102
Issue number4
Publication statusPublished - Apr 2011

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Polychlorinated Biphenyls
Tandem Mass Spectrometry
Liquid Chromatography
Cytochrome P-450 Enzyme System
hydroxide ion

All Science Journal Classification (ASJC) codes

  • Medicine(all)

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Measurement method for hydroxylated polychlorinated biphenyls in the blood of Yusho patients by liquid chromatography-electrospray tandem mass spectrometry. / Tobiishi, Kazuhiro; Todaka, Takashi; Hirakawa, Hironori; Hori, Tsuguhide; Kajiwara, Jumboku; Hirata, Teruaki; Iida, Takao; Hiroshi, Uchi; Furue, Masutaka.

In: Fukuoka Acta Medica, Vol. 102, No. 4, 04.2011, p. 153-158.

Research output: Contribution to journalArticle

Tobiishi, K, Todaka, T, Hirakawa, H, Hori, T, Kajiwara, J, Hirata, T, Iida, T, Hiroshi, U & Furue, M 2011, 'Measurement method for hydroxylated polychlorinated biphenyls in the blood of Yusho patients by liquid chromatography-electrospray tandem mass spectrometry.', Fukuoka Acta Medica, vol. 102, no. 4, pp. 153-158.
Tobiishi, Kazuhiro ; Todaka, Takashi ; Hirakawa, Hironori ; Hori, Tsuguhide ; Kajiwara, Jumboku ; Hirata, Teruaki ; Iida, Takao ; Hiroshi, Uchi ; Furue, Masutaka. / Measurement method for hydroxylated polychlorinated biphenyls in the blood of Yusho patients by liquid chromatography-electrospray tandem mass spectrometry. In: Fukuoka Acta Medica. 2011 ; Vol. 102, No. 4. pp. 153-158.
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