Measurement of adenylyl cyclase by separating cyclic AMP on silica gel thin-layer chromatography

Haruhiro Higashida; Kazi Z. Hossain; Hiroko Takahagi; Mami Noda

doi:10.1016/S0003-2697(02)00237-3

Measurement of adenylyl cyclase by separating cyclic AMP on silica gel thin-layer chromatography

Haruhiro Higashida, Kazi Z. Hossain, Hiroko Takahagi, Mami Noda

Pharmaceutical Health Care and Sciences Faculty

Research output: Contribution to journal › Article › peer-review

11 Citations (Scopus)

Abstract

A procedure for isolation of cyclic AMP (cAMP) by thin-layer chromatography on silica gel is described. One-dimensional ascending chromatograms were developed using [H₂O/C₂H₅OH/NH₄HCO₃ (30%:70%:0.2 M)] as the mobile phase. This procedure separated [³²P]cAMP from other radioactive metabolites of [³²P]ATP in up to 19 samples on one sheet (20 × 10cm) over 40-60 min at room temperature (21 °C). This simple and rapid isolation method provides a novel and convenient technique for the assay of adenylyl cyclase.

Original language	English
Pages (from-to)	106-111
Number of pages	6
Journal	Analytical Biochemistry
Volume	308
Issue number	1
DOIs	https://doi.org/10.1016/S0003-2697(02)00237-3
Publication status	Published - Sep 1 2002

All Science Journal Classification (ASJC) codes

Biophysics
Biochemistry
Molecular Biology
Cell Biology

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abstract = "A procedure for isolation of cyclic AMP (cAMP) by thin-layer chromatography on silica gel is described. One-dimensional ascending chromatograms were developed using [H2O/C2H5OH/NH4HCO3 (30%:70%:0.2 M)] as the mobile phase. This procedure separated [32P]cAMP from other radioactive metabolites of [32P]ATP in up to 19 samples on one sheet (20 × 10cm) over 40-60 min at room temperature (21 °C). This simple and rapid isolation method provides a novel and convenient technique for the assay of adenylyl cyclase.",

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AU - Higashida, Haruhiro

AU - Hossain, Kazi Z.

AU - Takahagi, Hiroko

AU - Noda, Mami

PY - 2002/9/1

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AB - A procedure for isolation of cyclic AMP (cAMP) by thin-layer chromatography on silica gel is described. One-dimensional ascending chromatograms were developed using [H2O/C2H5OH/NH4HCO3 (30%:70%:0.2 M)] as the mobile phase. This procedure separated [32P]cAMP from other radioactive metabolites of [32P]ATP in up to 19 samples on one sheet (20 × 10cm) over 40-60 min at room temperature (21 °C). This simple and rapid isolation method provides a novel and convenient technique for the assay of adenylyl cyclase.

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Measurement of adenylyl cyclase by separating cyclic AMP on silica gel thin-layer chromatography

Abstract

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