Measurement of adenylyl cyclase by separating cyclic AMP on silica gel thin-layer chromatography

Haruhiro Higashida, Kazi Z. Hossain, Hiroko Takahagi, Mami Noda

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

A procedure for isolation of cyclic AMP (cAMP) by thin-layer chromatography on silica gel is described. One-dimensional ascending chromatograms were developed using [H2O/C2H5OH/NH4HCO3 (30%:70%:0.2 M)] as the mobile phase. This procedure separated [32P]cAMP from other radioactive metabolites of [32P]ATP in up to 19 samples on one sheet (20 × 10cm) over 40-60 min at room temperature (21 °C). This simple and rapid isolation method provides a novel and convenient technique for the assay of adenylyl cyclase.

Original languageEnglish
Pages (from-to)106-111
Number of pages6
JournalAnalytical Biochemistry
Volume308
Issue number1
DOIs
Publication statusPublished - Sep 1 2002

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

Fingerprint Dive into the research topics of 'Measurement of adenylyl cyclase by separating cyclic AMP on silica gel thin-layer chromatography'. Together they form a unique fingerprint.

Cite this