Measurement of Forces between Surfaces Composed of Two-Dimensionally Organized, Complementary and Noncomplementary Nucleobases

Peter Berndt, Kazue Kurihara, Toyoki Kunitake

Research output: Contribution to journalArticle

47 Citations (Scopus)

Abstract

Langmuir-Blodgett films of amphiphiles with an orotic acid (Oro, uracil-like) headgroup or a complementary adenine (Ade) headgroup were deposited onto mica surfaces and investigated by surface force measurement. Interactions between both complementary (Oro-Ade) and noncomplementary (Ade-Ade, Oro-Oro) nucleobase pairs were studied. The LB films showed functional features of the nucleobase headgroups. Electrolyte-dependent electrostatic repulsion was found at pH's close to the pKa of the headgroups. In the absence of strong electrostatic forces, i.e., Oro-Ade and Oro-Oro combinations at pH 5.6, weak long-range attraction was detected. At close distances strong attractive forces made the surfaces jump into a contact position. The adhesive force between the complementary layers was found to be 100 mN/m, while the values between the noncomplementary pairs were ca. 50 mN/m and 250 ± 50 mN/m for Ade-Ade and Oro-Oro, respectively. The force profiles found between nucleobase surfaces are made of two components. One is observed at ca. 100-20 nm and changes its intensity drastically depending on conditions. This force is found both for complementary and for noncomplementary surfaces and is similar to the very long range attraction reported for hydrophobic surfaces (Claesson et al., 1988, 1994; Kurihara et al., 1990, 1992; Evans, 1993). The other, appearing at around 20 nm, is characteristic of complementary pairs and remains unchanged for pH changes and by salt (KBr) addition. These forces may have an important bearing on interaction mechanisms involved in molecular recognition and supramolecular assembling in biological systems.

Original languageEnglish
Pages (from-to)3083-3091
Number of pages9
JournalLangmuir
Volume11
Issue number8
DOIs
Publication statusPublished - Aug 1 1995

Fingerprint

Adenine
adenines
Langmuir Blodgett films
Molecular recognition
Amphiphiles
Electrostatic force
Force measurement
attraction
Surface measurement
Mica
Biological systems
Orotic Acid
electrostatics
Electrostatics
Uracil
Adhesives
uracil
Electrolytes
Salts
Langmuir-Blodgett films

All Science Journal Classification (ASJC) codes

  • Materials Science(all)
  • Condensed Matter Physics
  • Surfaces and Interfaces
  • Spectroscopy
  • Electrochemistry

Cite this

Measurement of Forces between Surfaces Composed of Two-Dimensionally Organized, Complementary and Noncomplementary Nucleobases. / Berndt, Peter; Kurihara, Kazue; Kunitake, Toyoki.

In: Langmuir, Vol. 11, No. 8, 01.08.1995, p. 3083-3091.

Research output: Contribution to journalArticle

Berndt, Peter ; Kurihara, Kazue ; Kunitake, Toyoki. / Measurement of Forces between Surfaces Composed of Two-Dimensionally Organized, Complementary and Noncomplementary Nucleobases. In: Langmuir. 1995 ; Vol. 11, No. 8. pp. 3083-3091.
@article{abd05c03fcd549e38099d6901040c1e9,
title = "Measurement of Forces between Surfaces Composed of Two-Dimensionally Organized, Complementary and Noncomplementary Nucleobases",
abstract = "Langmuir-Blodgett films of amphiphiles with an orotic acid (Oro, uracil-like) headgroup or a complementary adenine (Ade) headgroup were deposited onto mica surfaces and investigated by surface force measurement. Interactions between both complementary (Oro-Ade) and noncomplementary (Ade-Ade, Oro-Oro) nucleobase pairs were studied. The LB films showed functional features of the nucleobase headgroups. Electrolyte-dependent electrostatic repulsion was found at pH's close to the pKa of the headgroups. In the absence of strong electrostatic forces, i.e., Oro-Ade and Oro-Oro combinations at pH 5.6, weak long-range attraction was detected. At close distances strong attractive forces made the surfaces jump into a contact position. The adhesive force between the complementary layers was found to be 100 mN/m, while the values between the noncomplementary pairs were ca. 50 mN/m and 250 ± 50 mN/m for Ade-Ade and Oro-Oro, respectively. The force profiles found between nucleobase surfaces are made of two components. One is observed at ca. 100-20 nm and changes its intensity drastically depending on conditions. This force is found both for complementary and for noncomplementary surfaces and is similar to the very long range attraction reported for hydrophobic surfaces (Claesson et al., 1988, 1994; Kurihara et al., 1990, 1992; Evans, 1993). The other, appearing at around 20 nm, is characteristic of complementary pairs and remains unchanged for pH changes and by salt (KBr) addition. These forces may have an important bearing on interaction mechanisms involved in molecular recognition and supramolecular assembling in biological systems.",
author = "Peter Berndt and Kazue Kurihara and Toyoki Kunitake",
year = "1995",
month = "8",
day = "1",
doi = "10.1021/la00008a038",
language = "English",
volume = "11",
pages = "3083--3091",
journal = "Langmuir",
issn = "0743-7463",
publisher = "American Chemical Society",
number = "8",

}

TY - JOUR

T1 - Measurement of Forces between Surfaces Composed of Two-Dimensionally Organized, Complementary and Noncomplementary Nucleobases

AU - Berndt, Peter

AU - Kurihara, Kazue

AU - Kunitake, Toyoki

PY - 1995/8/1

Y1 - 1995/8/1

N2 - Langmuir-Blodgett films of amphiphiles with an orotic acid (Oro, uracil-like) headgroup or a complementary adenine (Ade) headgroup were deposited onto mica surfaces and investigated by surface force measurement. Interactions between both complementary (Oro-Ade) and noncomplementary (Ade-Ade, Oro-Oro) nucleobase pairs were studied. The LB films showed functional features of the nucleobase headgroups. Electrolyte-dependent electrostatic repulsion was found at pH's close to the pKa of the headgroups. In the absence of strong electrostatic forces, i.e., Oro-Ade and Oro-Oro combinations at pH 5.6, weak long-range attraction was detected. At close distances strong attractive forces made the surfaces jump into a contact position. The adhesive force between the complementary layers was found to be 100 mN/m, while the values between the noncomplementary pairs were ca. 50 mN/m and 250 ± 50 mN/m for Ade-Ade and Oro-Oro, respectively. The force profiles found between nucleobase surfaces are made of two components. One is observed at ca. 100-20 nm and changes its intensity drastically depending on conditions. This force is found both for complementary and for noncomplementary surfaces and is similar to the very long range attraction reported for hydrophobic surfaces (Claesson et al., 1988, 1994; Kurihara et al., 1990, 1992; Evans, 1993). The other, appearing at around 20 nm, is characteristic of complementary pairs and remains unchanged for pH changes and by salt (KBr) addition. These forces may have an important bearing on interaction mechanisms involved in molecular recognition and supramolecular assembling in biological systems.

AB - Langmuir-Blodgett films of amphiphiles with an orotic acid (Oro, uracil-like) headgroup or a complementary adenine (Ade) headgroup were deposited onto mica surfaces and investigated by surface force measurement. Interactions between both complementary (Oro-Ade) and noncomplementary (Ade-Ade, Oro-Oro) nucleobase pairs were studied. The LB films showed functional features of the nucleobase headgroups. Electrolyte-dependent electrostatic repulsion was found at pH's close to the pKa of the headgroups. In the absence of strong electrostatic forces, i.e., Oro-Ade and Oro-Oro combinations at pH 5.6, weak long-range attraction was detected. At close distances strong attractive forces made the surfaces jump into a contact position. The adhesive force between the complementary layers was found to be 100 mN/m, while the values between the noncomplementary pairs were ca. 50 mN/m and 250 ± 50 mN/m for Ade-Ade and Oro-Oro, respectively. The force profiles found between nucleobase surfaces are made of two components. One is observed at ca. 100-20 nm and changes its intensity drastically depending on conditions. This force is found both for complementary and for noncomplementary surfaces and is similar to the very long range attraction reported for hydrophobic surfaces (Claesson et al., 1988, 1994; Kurihara et al., 1990, 1992; Evans, 1993). The other, appearing at around 20 nm, is characteristic of complementary pairs and remains unchanged for pH changes and by salt (KBr) addition. These forces may have an important bearing on interaction mechanisms involved in molecular recognition and supramolecular assembling in biological systems.

UR - http://www.scopus.com/inward/record.url?scp=0000091998&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0000091998&partnerID=8YFLogxK

U2 - 10.1021/la00008a038

DO - 10.1021/la00008a038

M3 - Article

AN - SCOPUS:0000091998

VL - 11

SP - 3083

EP - 3091

JO - Langmuir

JF - Langmuir

SN - 0743-7463

IS - 8

ER -