Measurement of homogeneous kinase activity for cell lysates based on the aggregation of gold nanoparticles

Jun Oishi, Yoji Asami, Takeshi Mori, Jeong Hun Kang, Miharu Tanabe, Takuro Niidome, Yoshiki Katayama

    Research output: Contribution to journalArticlepeer-review

    70 Citations (Scopus)

    Abstract

    When the charge sticks. A kinase activity screening system based on the aggregation of gold nanoparticles induced by cationic substrate peptides as coagulants has been constructed. The system can conduct and monitor kinase reactions under homogeneous conditions and has been successfully applied to the kinase assay of cell lysates. (Figure Presented).

    Original languageEnglish
    Pages (from-to)875-879
    Number of pages5
    JournalChemBioChem
    Volume8
    Issue number8
    DOIs
    Publication statusPublished - May 25 2007

    All Science Journal Classification (ASJC) codes

    • Biochemistry
    • Molecular Medicine
    • Molecular Biology
    • Organic Chemistry

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