Measurement of intracellular free ca2+ concentration in guinea pig spiral ganglion cells

Masami Shimozono, Tetsuya Tono, Tamotsu Morimitsu, Takashi Nakagawa, Shizuo Komune

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9 Citations (Scopus)

Abstract

Intracellular free Ca2+ concentration ([Ca2+];) in spiral ganglion cells (SGC) of guinea pig was measured using the fura-2 fluorescence method. Application of high-K+ external solution increased [Ca2+]i. [Ca2+]i elevation was suppressed by either depletion of external Ca2+ or application of the dihydropyridine Ca2+ channel antagonist, nicardipine. One of the putative hair cell afferent nerve neurotransmitters, L-glutamate (Glu), induced [Ca2+]i elevation in SGC. Pharmacological studies suggested that the Glu receptor may be a non-NMDA subtype. Glu-induced response was suppressed by either depletion of external Ca2+ or application of nicardipine, indicating that Glu-induced [Ca2+]i elevation is mainly carried out by the voltage- dependent Ca2+ channel in SGC.

Original languageEnglish
Pages (from-to)421-424
Number of pages4
JournalNeuroReport
Volume6
Issue number3
DOIs
Publication statusPublished - Feb 1995

All Science Journal Classification (ASJC) codes

  • Neuroscience(all)

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