Measurement of intracellular free ca 2+ concentration in guinea pig spiral ganglion cells

Masami Shimozono, Tetsuya Tono, Tamotsu Morimitsu, Takashi Nakagawa, Shizuo Komune

Research output: Contribution to journalArticle

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Abstract

Intracellular free Ca 2+ concentration ([Ca 2+ ];) in spiral ganglion cells (SGC) of guinea pig was measured using the fura-2 fluorescence method. Application of high-K + external solution increased [Ca 2+ ] i . [Ca 2+ ] i elevation was suppressed by either depletion of external Ca 2+ or application of the dihydropyridine Ca 2+ channel antagonist, nicardipine. One of the putative hair cell afferent nerve neurotransmitters, L-glutamate (Glu), induced [Ca 2+ ] i elevation in SGC. Pharmacological studies suggested that the Glu receptor may be a non-NMDA subtype. Glu-induced response was suppressed by either depletion of external Ca 2+ or application of nicardipine, indicating that Glu-induced [Ca 2+ ] i elevation is mainly carried out by the voltage- dependent Ca 2+ channel in SGC.

Original languageEnglish
Pages (from-to)421-424
Number of pages4
JournalNeuroReport
Volume6
Issue number3
DOIs
Publication statusPublished - Jan 1 1995

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Spiral Ganglion
Nicardipine
Glutamic Acid
Guinea Pigs
Fura-2
Glutamate Receptors
Neurotransmitter Agents
Fluorescence
Pharmacology
Neurons

All Science Journal Classification (ASJC) codes

  • Neuroscience(all)

Cite this

Measurement of intracellular free ca 2+ concentration in guinea pig spiral ganglion cells . / Shimozono, Masami; Tono, Tetsuya; Morimitsu, Tamotsu; Nakagawa, Takashi; Komune, Shizuo.

In: NeuroReport, Vol. 6, No. 3, 01.01.1995, p. 421-424.

Research output: Contribution to journalArticle

Shimozono, Masami ; Tono, Tetsuya ; Morimitsu, Tamotsu ; Nakagawa, Takashi ; Komune, Shizuo. / Measurement of intracellular free ca 2+ concentration in guinea pig spiral ganglion cells In: NeuroReport. 1995 ; Vol. 6, No. 3. pp. 421-424.
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