A new method is proposed to measure the viability of round cells compressed by two parallel plates which simulate ice crystals during freezing of cell suspension. The viability of prostate cancer cells (cell line ND-1, 20 μm in mean diameter) is evaluated at about 23'C with trypan blue dye exclusion assay. While most of the cells survive deformation in a gap of 11.4 μm, about half of the cells are destroyed when the gap size is reduced to 5.9 μm, i.e. 30% of its original diameter. If uniform expansion of cell membrane is assumed, this corresponds to 50% increase in the cell membrane surface area.
|Number of pages||4|
|Journal||Nihon Kikai Gakkai Ronbunshu, B Hen/Transactions of the Japan Society of Mechanical Engineers, Part B|
|Publication status||Published - Nov 1999|
All Science Journal Classification (ASJC) codes
- Condensed Matter Physics
- Mechanical Engineering