Measurement of the viability of cells compressed by two parallel plates

Hiroshi Takamatsu; Boris Rubinsky

doi:10.1299/kikaib.65.3821

Measurement of the viability of cells compressed by two parallel plates

Hiroshi Takamatsu, Boris Rubinsky

Thermal Engineering

Research output: Contribution to journal › Article › peer-review

Abstract

A new method is proposed to measure the viability of round cells compressed by two parallel plates which simulate ice crystals during freezing of cell suspension. The viability of prostate cancer cells (cell line ND-1, 20 μm in mean diameter) is evaluated at about 23'C with trypan blue dye exclusion assay. While most of the cells survive deformation in a gap of 11.4 μm, about half of the cells are destroyed when the gap size is reduced to 5.9 μm, i.e. 30% of its original diameter. If uniform expansion of cell membrane is assumed, this corresponds to 50% increase in the cell membrane surface area.

Original language	English
Pages (from-to)	3821-3824
Number of pages	4
Journal	Nihon Kikai Gakkai Ronbunshu, B Hen/Transactions of the Japan Society of Mechanical Engineers, Part B
Volume	65
Issue number	639
DOIs	https://doi.org/10.1299/kikaib.65.3821
Publication status	Published - Nov 1999

All Science Journal Classification (ASJC) codes

Condensed Matter Physics
Mechanical Engineering

Access to Document

10.1299/kikaib.65.3821

Fingerprint Dive into the research topics of 'Measurement of the viability of cells compressed by two parallel plates'. Together they form a unique fingerprint.

Cite this

@article{4c0acb43e28e448dac4c065e28e6fb32,

title = "Measurement of the viability of cells compressed by two parallel plates",

abstract = "A new method is proposed to measure the viability of round cells compressed by two parallel plates which simulate ice crystals during freezing of cell suspension. The viability of prostate cancer cells (cell line ND-1, 20 μm in mean diameter) is evaluated at about 23'C with trypan blue dye exclusion assay. While most of the cells survive deformation in a gap of 11.4 μm, about half of the cells are destroyed when the gap size is reduced to 5.9 μm, i.e. 30% of its original diameter. If uniform expansion of cell membrane is assumed, this corresponds to 50% increase in the cell membrane surface area.",

author = "Hiroshi Takamatsu and Boris Rubinsky",

year = "1999",

month = nov,

doi = "10.1299/kikaib.65.3821",

language = "English",

volume = "65",

pages = "3821--3824",

journal = "Nihon Kikai Gakkai Ronbunshu, B Hen/Transactions of the Japan Society of Mechanical Engineers, Part B",

issn = "0387-5016",

publisher = "The Japan Society of Mechanical Engineers",

number = "639",

}

TY - JOUR

T1 - Measurement of the viability of cells compressed by two parallel plates

AU - Takamatsu, Hiroshi

AU - Rubinsky, Boris

PY - 1999/11

Y1 - 1999/11

N2 - A new method is proposed to measure the viability of round cells compressed by two parallel plates which simulate ice crystals during freezing of cell suspension. The viability of prostate cancer cells (cell line ND-1, 20 μm in mean diameter) is evaluated at about 23'C with trypan blue dye exclusion assay. While most of the cells survive deformation in a gap of 11.4 μm, about half of the cells are destroyed when the gap size is reduced to 5.9 μm, i.e. 30% of its original diameter. If uniform expansion of cell membrane is assumed, this corresponds to 50% increase in the cell membrane surface area.

AB - A new method is proposed to measure the viability of round cells compressed by two parallel plates which simulate ice crystals during freezing of cell suspension. The viability of prostate cancer cells (cell line ND-1, 20 μm in mean diameter) is evaluated at about 23'C with trypan blue dye exclusion assay. While most of the cells survive deformation in a gap of 11.4 μm, about half of the cells are destroyed when the gap size is reduced to 5.9 μm, i.e. 30% of its original diameter. If uniform expansion of cell membrane is assumed, this corresponds to 50% increase in the cell membrane surface area.

UR - http://www.scopus.com/inward/record.url?scp=71449115748&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=71449115748&partnerID=8YFLogxK

U2 - 10.1299/kikaib.65.3821

DO - 10.1299/kikaib.65.3821

M3 - Article

AN - SCOPUS:71449115748

VL - 65

SP - 3821

EP - 3824

JO - Nihon Kikai Gakkai Ronbunshu, B Hen/Transactions of the Japan Society of Mechanical Engineers, Part B

JF - Nihon Kikai Gakkai Ronbunshu, B Hen/Transactions of the Japan Society of Mechanical Engineers, Part B

SN - 0387-5016

IS - 639

ER -

Measurement of the viability of cells compressed by two parallel plates

Abstract

All Science Journal Classification (ASJC) codes

Access to Document

Other files and links

Cite this