Mechanical characterization of adult stem cells from bone marrow and perivascular niches

Alexandre J.S. Ribeiro, Steven Tottey, Richard W.E. Taylor, Ryoma Bise, Takeo Kanade, Stephen F. Badylak, Kris Noel Dahl

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

Therapies using adult stem cells often require mechanical manipulation such as injection or incorporation into scaffolds. However, force-induced rupture and mechanosensitivity of cells during manipulation is largely ignored. Here, we image cell mechanical structures and perform a biophysical characterization of three different types of human adult stem cells: bone marrow CD34+ hematopoietic, bone marrow mesenchymal and perivascular mesenchymal stem cells. We use micropipette aspiration to characterize cell mechanics and quantify deformation of subcellular structures under force and its contribution to global cell deformation. Our results suggest that CD34+ cells are mechanically suitable for injection systems since cells transition from solid- to fluid-like at constant aspiration pressure, probably due to a poorly developed actin cytoskeleton. Conversely, mesenchymal stem cells from the bone marrow and perivascular niches are more suitable for seeding into biomaterial scaffolds since they are mechanically robust and have developed cytoskeletal structures that may allow cellular stable attachment and motility through solid porous environments. Among these, perivascular stem cells cultured in 6% oxygen show a developed cytoskeleton but a more compliant nucleus, which can facilitate the penetration into pores of tissues or scaffolds. We confirm the relevance of our measurements using cell motility and migration assays and measure survival of injected cells. Since different types of adult stem cells can be used for similar applications, we suggest considering mechanical properties of stem cells to match optimal mechanical characteristics of therapies.

Original languageEnglish
Pages (from-to)1280-1287
Number of pages8
JournalJournal of Biomechanics
Volume45
Issue number7
DOIs
Publication statusPublished - Apr 30 2012

Fingerprint

Adult Stem Cells
Stem cells
Bone
Bone Marrow
Cell Migration Assays
Scaffolds
Mesenchymal Stromal Cells
Stem Cells
Tissue Scaffolds
Injections
Biocompatible Materials
Mechanics
Cytoskeleton
Actin Cytoskeleton
Rupture
Cell Survival
Biomaterials
Assays
Oxygen
Pressure

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Orthopedics and Sports Medicine
  • Biomedical Engineering
  • Rehabilitation

Cite this

Ribeiro, A. J. S., Tottey, S., Taylor, R. W. E., Bise, R., Kanade, T., Badylak, S. F., & Dahl, K. N. (2012). Mechanical characterization of adult stem cells from bone marrow and perivascular niches. Journal of Biomechanics, 45(7), 1280-1287. https://doi.org/10.1016/j.jbiomech.2012.01.032

Mechanical characterization of adult stem cells from bone marrow and perivascular niches. / Ribeiro, Alexandre J.S.; Tottey, Steven; Taylor, Richard W.E.; Bise, Ryoma; Kanade, Takeo; Badylak, Stephen F.; Dahl, Kris Noel.

In: Journal of Biomechanics, Vol. 45, No. 7, 30.04.2012, p. 1280-1287.

Research output: Contribution to journalArticle

Ribeiro, AJS, Tottey, S, Taylor, RWE, Bise, R, Kanade, T, Badylak, SF & Dahl, KN 2012, 'Mechanical characterization of adult stem cells from bone marrow and perivascular niches', Journal of Biomechanics, vol. 45, no. 7, pp. 1280-1287. https://doi.org/10.1016/j.jbiomech.2012.01.032
Ribeiro, Alexandre J.S. ; Tottey, Steven ; Taylor, Richard W.E. ; Bise, Ryoma ; Kanade, Takeo ; Badylak, Stephen F. ; Dahl, Kris Noel. / Mechanical characterization of adult stem cells from bone marrow and perivascular niches. In: Journal of Biomechanics. 2012 ; Vol. 45, No. 7. pp. 1280-1287.
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