Mechanical stress accompanied with nuclear rotation is involved in the dormant state of mouse oocytes

Go Nagamatsu, So Shimamoto, Nobuhiko Hamazaki, Yohei Nishimura, Katsuhiko Hayashi

Research output: Contribution to journalArticlepeer-review

11 Citations (Scopus)

Abstract

The most immature oocytes remain dormant in primordial follicles in the ovary, ensuring the longevity of female reproductive life. Despite its biological and clinical importance, knowledge of mechanisms regulating the dormant state remains limited. Here, we show that mechanical stress plays a key role in maintaining the dormant state of the oocytes in primordial follicles in mice. Transcriptional and histological analyses revealed that oocytes were compressed by surrounding granulosa cells with extracellular matrix. This environmental state is functionally crucial, as oocytes became activated upon loosening the structure and the dormancy was restored by additional compression with exogenous pressure. The nuclei of oocytes in primordial follicles rotated in response to the mechanical stress. Pausing the rotation triggered activation of oocytes through nuclear export of forkhead box O3 (FOXO3). These results provide insights into the mechanisms by which oocytes are kept dormant to sustain female reproductive life.

Original languageEnglish
Article numbereaav9960
JournalScience Advances
Volume5
Issue number6
DOIs
Publication statusPublished - 2019

All Science Journal Classification (ASJC) codes

  • Physics and Astronomy (miscellaneous)
  • General

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