Mechanical stress-related calvaria bone augmentation by onlayed octacalcium phosphate-collagen implant

Aritsune Matsui, Takahisa Anada, Taisuke Masuda, Yoshitomo Honda, Naohisa Miyatake, Tadashi Kawai, Shinji Kamakura, Seishi Echigo, Osamu Suzuki

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

Previous studies have suggested that the biodegradability of octacalcium phosphate-collagen (OCP/Col) composite by osteoclasts is accelerated in association with mechanical stress suffered by the host tissue around the implant. The present study was designed to investigate whether alleviation of mechanical stress restores the bone regenerative properties of OCP/Col, as previously shown in nonload-bearing sites. OCP/Col discs supported with a polytetrafluoroethylene (PTFE) ring, which has a higher modulus than OCP/Col, were implanted in a rat subperiosteal pocket for up to 12 weeks. The structural features of the implant and biological responses were analyzed by X-ray diffraction, Fourier transform infrared spectroscopy, histomorphometry, histochemistry, and tissue mRNA expression around the implants. The effect of compression was analyzed using mouse stromal ST-2 cells by deforming the cell-seeded OCP/Col discs in vitro with or without a PTFE ring. The results clearly indicated the restoration of bone formation by the alleviation of mechanical stress and the upregulation of osteoblast-related genes, such as osterix on the other hand, the implantation of OCP/Col on calvaria or in an in vitro test without PTFE support resulted in the upregulation of osteoclast-related genes, such as tartrate-resistant acid phosphatase (TRAP) and cathepsin K, in the tissues or receptor activator of the nuclear factor-κB ligand (RANKL) in ST-2 cells. The results confirmed that calvaria augmentation is enhanced by implanting OCP/Col if suitable conditions regarding mechanical stress are provided.

Original languageEnglish
Pages (from-to)139-151
Number of pages13
JournalTissue Engineering - Part A
Volume16
Issue number1
DOIs
Publication statusPublished - Jan 1 2010
Externally publishedYes

Fingerprint

Mechanical Stress
Collagen
Skull
Bone
Phosphates
Bone and Bones
Polytetrafluoroethylene
Polytetrafluoroethylenes
Osteoclasts
Tissue
Bearings (structural)
Up-Regulation
Genes
Cathepsin K
Biodegradability
Osteoblasts
Phosphatases
Fourier Transform Infrared Spectroscopy
Cytoplasmic and Nuclear Receptors
Acid Phosphatase

All Science Journal Classification (ASJC) codes

  • Bioengineering
  • Biochemistry
  • Biomedical Engineering
  • Biomaterials
  • Medicine(all)

Cite this

Mechanical stress-related calvaria bone augmentation by onlayed octacalcium phosphate-collagen implant. / Matsui, Aritsune; Anada, Takahisa; Masuda, Taisuke; Honda, Yoshitomo; Miyatake, Naohisa; Kawai, Tadashi; Kamakura, Shinji; Echigo, Seishi; Suzuki, Osamu.

In: Tissue Engineering - Part A, Vol. 16, No. 1, 01.01.2010, p. 139-151.

Research output: Contribution to journalArticle

Matsui, A, Anada, T, Masuda, T, Honda, Y, Miyatake, N, Kawai, T, Kamakura, S, Echigo, S & Suzuki, O 2010, 'Mechanical stress-related calvaria bone augmentation by onlayed octacalcium phosphate-collagen implant', Tissue Engineering - Part A, vol. 16, no. 1, pp. 139-151. https://doi.org/10.1089/ten.tea.2009.0284
Matsui, Aritsune ; Anada, Takahisa ; Masuda, Taisuke ; Honda, Yoshitomo ; Miyatake, Naohisa ; Kawai, Tadashi ; Kamakura, Shinji ; Echigo, Seishi ; Suzuki, Osamu. / Mechanical stress-related calvaria bone augmentation by onlayed octacalcium phosphate-collagen implant. In: Tissue Engineering - Part A. 2010 ; Vol. 16, No. 1. pp. 139-151.
@article{f009540111a34e418a6ecf4c56c93fe5,
title = "Mechanical stress-related calvaria bone augmentation by onlayed octacalcium phosphate-collagen implant",
abstract = "Previous studies have suggested that the biodegradability of octacalcium phosphate-collagen (OCP/Col) composite by osteoclasts is accelerated in association with mechanical stress suffered by the host tissue around the implant. The present study was designed to investigate whether alleviation of mechanical stress restores the bone regenerative properties of OCP/Col, as previously shown in nonload-bearing sites. OCP/Col discs supported with a polytetrafluoroethylene (PTFE) ring, which has a higher modulus than OCP/Col, were implanted in a rat subperiosteal pocket for up to 12 weeks. The structural features of the implant and biological responses were analyzed by X-ray diffraction, Fourier transform infrared spectroscopy, histomorphometry, histochemistry, and tissue mRNA expression around the implants. The effect of compression was analyzed using mouse stromal ST-2 cells by deforming the cell-seeded OCP/Col discs in vitro with or without a PTFE ring. The results clearly indicated the restoration of bone formation by the alleviation of mechanical stress and the upregulation of osteoblast-related genes, such as osterix on the other hand, the implantation of OCP/Col on calvaria or in an in vitro test without PTFE support resulted in the upregulation of osteoclast-related genes, such as tartrate-resistant acid phosphatase (TRAP) and cathepsin K, in the tissues or receptor activator of the nuclear factor-κB ligand (RANKL) in ST-2 cells. The results confirmed that calvaria augmentation is enhanced by implanting OCP/Col if suitable conditions regarding mechanical stress are provided.",
author = "Aritsune Matsui and Takahisa Anada and Taisuke Masuda and Yoshitomo Honda and Naohisa Miyatake and Tadashi Kawai and Shinji Kamakura and Seishi Echigo and Osamu Suzuki",
year = "2010",
month = "1",
day = "1",
doi = "10.1089/ten.tea.2009.0284",
language = "English",
volume = "16",
pages = "139--151",
journal = "Tissue Engineering - Part A.",
issn = "1937-3341",
publisher = "Mary Ann Liebert Inc.",
number = "1",

}

TY - JOUR

T1 - Mechanical stress-related calvaria bone augmentation by onlayed octacalcium phosphate-collagen implant

AU - Matsui, Aritsune

AU - Anada, Takahisa

AU - Masuda, Taisuke

AU - Honda, Yoshitomo

AU - Miyatake, Naohisa

AU - Kawai, Tadashi

AU - Kamakura, Shinji

AU - Echigo, Seishi

AU - Suzuki, Osamu

PY - 2010/1/1

Y1 - 2010/1/1

N2 - Previous studies have suggested that the biodegradability of octacalcium phosphate-collagen (OCP/Col) composite by osteoclasts is accelerated in association with mechanical stress suffered by the host tissue around the implant. The present study was designed to investigate whether alleviation of mechanical stress restores the bone regenerative properties of OCP/Col, as previously shown in nonload-bearing sites. OCP/Col discs supported with a polytetrafluoroethylene (PTFE) ring, which has a higher modulus than OCP/Col, were implanted in a rat subperiosteal pocket for up to 12 weeks. The structural features of the implant and biological responses were analyzed by X-ray diffraction, Fourier transform infrared spectroscopy, histomorphometry, histochemistry, and tissue mRNA expression around the implants. The effect of compression was analyzed using mouse stromal ST-2 cells by deforming the cell-seeded OCP/Col discs in vitro with or without a PTFE ring. The results clearly indicated the restoration of bone formation by the alleviation of mechanical stress and the upregulation of osteoblast-related genes, such as osterix on the other hand, the implantation of OCP/Col on calvaria or in an in vitro test without PTFE support resulted in the upregulation of osteoclast-related genes, such as tartrate-resistant acid phosphatase (TRAP) and cathepsin K, in the tissues or receptor activator of the nuclear factor-κB ligand (RANKL) in ST-2 cells. The results confirmed that calvaria augmentation is enhanced by implanting OCP/Col if suitable conditions regarding mechanical stress are provided.

AB - Previous studies have suggested that the biodegradability of octacalcium phosphate-collagen (OCP/Col) composite by osteoclasts is accelerated in association with mechanical stress suffered by the host tissue around the implant. The present study was designed to investigate whether alleviation of mechanical stress restores the bone regenerative properties of OCP/Col, as previously shown in nonload-bearing sites. OCP/Col discs supported with a polytetrafluoroethylene (PTFE) ring, which has a higher modulus than OCP/Col, were implanted in a rat subperiosteal pocket for up to 12 weeks. The structural features of the implant and biological responses were analyzed by X-ray diffraction, Fourier transform infrared spectroscopy, histomorphometry, histochemistry, and tissue mRNA expression around the implants. The effect of compression was analyzed using mouse stromal ST-2 cells by deforming the cell-seeded OCP/Col discs in vitro with or without a PTFE ring. The results clearly indicated the restoration of bone formation by the alleviation of mechanical stress and the upregulation of osteoblast-related genes, such as osterix on the other hand, the implantation of OCP/Col on calvaria or in an in vitro test without PTFE support resulted in the upregulation of osteoclast-related genes, such as tartrate-resistant acid phosphatase (TRAP) and cathepsin K, in the tissues or receptor activator of the nuclear factor-κB ligand (RANKL) in ST-2 cells. The results confirmed that calvaria augmentation is enhanced by implanting OCP/Col if suitable conditions regarding mechanical stress are provided.

UR - http://www.scopus.com/inward/record.url?scp=77049094276&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=77049094276&partnerID=8YFLogxK

U2 - 10.1089/ten.tea.2009.0284

DO - 10.1089/ten.tea.2009.0284

M3 - Article

C2 - 19642866

AN - SCOPUS:77049094276

VL - 16

SP - 139

EP - 151

JO - Tissue Engineering - Part A.

JF - Tissue Engineering - Part A.

SN - 1937-3341

IS - 1

ER -