Mechanical variation and proliferation behavior in hydroxyapatite based scaffolds with mesenchymal stem cells

Phanny Yos, Md Abdul Kafi, Mitsugu Todo

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

Calcium phosphate bioceramics such as hydroxyapatite (HA) have widely been applied as scaffolds in bone tissue engineering because of high osteo-conductivity and biocompatibility. In the present study, continuous porous HA scaffold was fabricated using the template method. Human mesenchymal stem cells (hMSC) were then seeded into the HA scaffold up to four weeks to observe the proliferation behavior and variation of the compressive mechanical properties. The scaffold with hMSCs was also characterized by scanning electron microscopy (SEM). It was found that the compressive strength and elastic modulus tend to increase with increasing culture time due to proliferation and attachment of the cells. The HA scaffold was also found to be suitable for hMSC adhesion, spreading and proliferation. Moreover, improvement of cellular adhesion was achieved by introducing RGD (Arg-Gly- Asp) peptide into the HA scaffold. We can conclude that the HA scaffold provides good environmental conditions for hMSCs as an artificial extracellular matrix. It is also important to note that the cellular adhesion can be effectively improved by RGD.

Original languageEnglish
Title of host publicationThe 15th International Conference on Biomedical Engineering, ICBME 2013
EditorsJames Goh
PublisherSpringer Verlag
Pages283-286
Number of pages4
Volume43
ISBN (Electronic)9783319029122
DOIs
Publication statusPublished - Jan 1 2014
Event15th International Conference on Biomedical Engineering, ICBME 2013 - Singapore, Singapore
Duration: Dec 4 2013Dec 7 2013

Other

Other15th International Conference on Biomedical Engineering, ICBME 2013
CountrySingapore
CitySingapore
Period12/4/1312/7/13

Fingerprint

Durapatite
Stem cells
Hydroxyapatite
Scaffolds
Scaffolds (biology)
Adhesion
Bioceramics
Cell adhesion
Cell proliferation
Calcium phosphate
Biocompatibility
Tissue engineering
Peptides
Compressive strength
Bone
Elastic moduli
Mechanical properties
Scanning electron microscopy

All Science Journal Classification (ASJC) codes

  • Bioengineering
  • Biomedical Engineering

Cite this

Yos, P., Kafi, M. A., & Todo, M. (2014). Mechanical variation and proliferation behavior in hydroxyapatite based scaffolds with mesenchymal stem cells. In J. Goh (Ed.), The 15th International Conference on Biomedical Engineering, ICBME 2013 (Vol. 43, pp. 283-286). Springer Verlag. https://doi.org/10.1007/978-3-319-02913-9_72

Mechanical variation and proliferation behavior in hydroxyapatite based scaffolds with mesenchymal stem cells. / Yos, Phanny; Kafi, Md Abdul; Todo, Mitsugu.

The 15th International Conference on Biomedical Engineering, ICBME 2013. ed. / James Goh. Vol. 43 Springer Verlag, 2014. p. 283-286.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Yos, P, Kafi, MA & Todo, M 2014, Mechanical variation and proliferation behavior in hydroxyapatite based scaffolds with mesenchymal stem cells. in J Goh (ed.), The 15th International Conference on Biomedical Engineering, ICBME 2013. vol. 43, Springer Verlag, pp. 283-286, 15th International Conference on Biomedical Engineering, ICBME 2013, Singapore, Singapore, 12/4/13. https://doi.org/10.1007/978-3-319-02913-9_72
Yos P, Kafi MA, Todo M. Mechanical variation and proliferation behavior in hydroxyapatite based scaffolds with mesenchymal stem cells. In Goh J, editor, The 15th International Conference on Biomedical Engineering, ICBME 2013. Vol. 43. Springer Verlag. 2014. p. 283-286 https://doi.org/10.1007/978-3-319-02913-9_72
Yos, Phanny ; Kafi, Md Abdul ; Todo, Mitsugu. / Mechanical variation and proliferation behavior in hydroxyapatite based scaffolds with mesenchymal stem cells. The 15th International Conference on Biomedical Engineering, ICBME 2013. editor / James Goh. Vol. 43 Springer Verlag, 2014. pp. 283-286
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