Mechanically Distinct Microtubule Arrays Determine the Length and Force Response of the Meiotic Spindle

Jun Takagi, Ryota Sakamoto, Gen Shiratsuchi, Yusuke Maeda, Yuta Shimamoto

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

The microtubule-based spindle is subjected to various mechanical forces during cell division. How the structure generates and responds to forces while maintaining overall integrity is unknown because we have a poor understanding of the relationship between filament architecture and mechanics. Here, to fill this gap, we combine microneedle-based quantitative micromanipulation with high-resolution imaging, simultaneously analyzing forces and local filament motility in the Xenopus meiotic spindle. We find that microtubules exhibit a compliant, fluid-like mechanical response at the middle of the spindle half, being distinct from those near the pole and the equator. A force altering spindle length induces filament sliding at this compliant array, where parallel microtubules predominate, without influencing equatorial antiparallel filament dynamics. Molecular perturbations suggest that kinesin-5 and dynein contribute to the spindle's local mechanical difference. Together, our data establish a link between spindle architecture and mechanics and uncover the mechanical design of this essential cytoskeletal assembly.

Original languageEnglish
Pages (from-to)267-278.e5
JournalDevelopmental Cell
Volume49
Issue number2
DOIs
Publication statusPublished - Apr 22 2019

Fingerprint

Spindle Apparatus
Microtubules
Mechanics
Dyneins
Kinesin
Micromanipulation
Poles
Cells
Xenopus
Imaging techniques
Cell Division
Fluids

All Science Journal Classification (ASJC) codes

  • Molecular Biology
  • Biochemistry, Genetics and Molecular Biology(all)
  • Developmental Biology
  • Cell Biology

Cite this

Mechanically Distinct Microtubule Arrays Determine the Length and Force Response of the Meiotic Spindle. / Takagi, Jun; Sakamoto, Ryota; Shiratsuchi, Gen; Maeda, Yusuke; Shimamoto, Yuta.

In: Developmental Cell, Vol. 49, No. 2, 22.04.2019, p. 267-278.e5.

Research output: Contribution to journalArticle

Takagi, Jun ; Sakamoto, Ryota ; Shiratsuchi, Gen ; Maeda, Yusuke ; Shimamoto, Yuta. / Mechanically Distinct Microtubule Arrays Determine the Length and Force Response of the Meiotic Spindle. In: Developmental Cell. 2019 ; Vol. 49, No. 2. pp. 267-278.e5.
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