Mechanism for the antibacterial action of epigallocatechin gallate (EGCg) on Bacillus subtilis

Motokazu Nakayama, Kanami Shimatani, Tadahiro Ozawa, Naofumi Shigemune, Daisuke Tomiyama, Koji Yui, Mao Katsuki, Keisuke Ikeda, Ai Nonaka, Takahisa Miyamoto

Research output: Contribution to journalArticle

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Abstract

Catechins are a class of polyphenols and have high anti-bacterial activity against various microorganisms. Here, we report the mechanism for antibacterial activity of epigallocatechin gallate (EGCg) against Gram-positive bacteria Bacillus subtilis, which is highly sensitive to EGCg. Transmission electron microscope analysis revealed that deposits containing EGCg were found throughout the cell envelope from the outermost surface to the outer surface of cytoplasmic membrane. Aggregating forms of proteins and EGCg were identified as spots that disappeared or showed markedly decreased intensity after the treatment with EGCg compared to the control by two-dimensional electrophoresis. Among the identified proteins included 4 cell surface proteins, such as oligopeptide ABC transporter binding lipoprotein, glucose phosphotransferase system transporter protein, phosphate ABC transporter substrate-binding protein, and penicillin- binding protein 5. Observations of glucose uptake of cells and cell shape B. subtilis after the treatment with EGCg suggested that EGCg inhibits the major functions of these proteins, leading to growth inhibition of B. subtilis.

Original languageEnglish
Pages (from-to)845-854
Number of pages10
JournalBioscience, Biotechnology and Biochemistry
Volume79
Issue number5
DOIs
Publication statusPublished - Jan 1 2015

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Bacilli
Bacillus subtilis
ATP-Binding Cassette Transporters
Proteins
Phosphate Transport Proteins
Penicillin-Binding Proteins
Glucose
Oligopeptides
Cell Shape
Catechin
Gram-Positive Bacteria
Polyphenols
epigallocatechin gallate
Electrophoresis
Microorganisms
Lipoproteins
Bacteria
Carrier Proteins
Membrane Proteins
Phosphotransferases

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Analytical Chemistry
  • Biochemistry
  • Applied Microbiology and Biotechnology
  • Molecular Biology
  • Organic Chemistry

Cite this

Mechanism for the antibacterial action of epigallocatechin gallate (EGCg) on Bacillus subtilis. / Nakayama, Motokazu; Shimatani, Kanami; Ozawa, Tadahiro; Shigemune, Naofumi; Tomiyama, Daisuke; Yui, Koji; Katsuki, Mao; Ikeda, Keisuke; Nonaka, Ai; Miyamoto, Takahisa.

In: Bioscience, Biotechnology and Biochemistry, Vol. 79, No. 5, 01.01.2015, p. 845-854.

Research output: Contribution to journalArticle

Nakayama, M, Shimatani, K, Ozawa, T, Shigemune, N, Tomiyama, D, Yui, K, Katsuki, M, Ikeda, K, Nonaka, A & Miyamoto, T 2015, 'Mechanism for the antibacterial action of epigallocatechin gallate (EGCg) on Bacillus subtilis', Bioscience, Biotechnology and Biochemistry, vol. 79, no. 5, pp. 845-854. https://doi.org/10.1080/09168451.2014.993356
Nakayama, Motokazu ; Shimatani, Kanami ; Ozawa, Tadahiro ; Shigemune, Naofumi ; Tomiyama, Daisuke ; Yui, Koji ; Katsuki, Mao ; Ikeda, Keisuke ; Nonaka, Ai ; Miyamoto, Takahisa. / Mechanism for the antibacterial action of epigallocatechin gallate (EGCg) on Bacillus subtilis. In: Bioscience, Biotechnology and Biochemistry. 2015 ; Vol. 79, No. 5. pp. 845-854.
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