Mechanism for the detoxification of aluminum in roots of tea plant (Camellia sinensis (L.) Kuntze)

Akio Morita, Osamu Yanagisawa, Satoshi Takatsu, Setsuko Maeda, Syuntaro Hiradate

Research output: Contribution to journalArticle

58 Citations (Scopus)

Abstract

To determine the mechanism of aluminum (Al) detoxification in the roots of tea plants (Camellia sinensis (L.) Kuntze), the amounts of Al and Al-chelating compounds (fluoride (F), organic acids and catechins) were measured and the chemical forms of Al in root cell extracts were identified by the application of 27Al-nuclear magnetic resonance (NMR) spectroscopy. Tea plants were cultivated in nutrient solutions containing 0, 4, 1.0 and 4.0 mM of Al at pH 4.2 for approximately 10 weeks. The levels of soluble Al, water-soluble oxalate and citrate, but not F, malate or catechins in young roots increased with an increase in the concentration of Al in the treatment solution. The 27Al NMR spectra of root tips and cell sap extracted from root tips that had been treated with Al were almost identical and had four signals, with two (11 and 16 ppm) apparently corresponding to the known chemical shifts of Al-oxalate complexes. In the spectra of cell sap, the resonances at 11 and 16 ppm increased with an increase in the Al contents. These results suggest that the levels of Al-oxalate complexes increased in response to an increase in the Al level, implying that oxalate is a key Al-chelating compound in the mechanism of Al detoxification in the tea root.

Original languageEnglish
Pages (from-to)147-153
Number of pages7
JournalPhytochemistry
Volume69
Issue number1
DOIs
Publication statusPublished - Jan 1 2008
Externally publishedYes

Fingerprint

Camellia sinensis
Detoxification
Plant Roots
Tea
Aluminum
tea
aluminum
Aluminum Compounds
oxalates
Oxalic Acid
Oxalates
Meristem
Catechin
Chelation
Magnetic Resonance Spectroscopy
flavanols
root tips
sap
nuclear magnetic resonance spectroscopy
Cell Extracts

All Science Journal Classification (ASJC) codes

  • Plant Science
  • Biochemistry
  • Molecular Biology
  • Organic Chemistry
  • Drug Discovery

Cite this

Mechanism for the detoxification of aluminum in roots of tea plant (Camellia sinensis (L.) Kuntze). / Morita, Akio; Yanagisawa, Osamu; Takatsu, Satoshi; Maeda, Setsuko; Hiradate, Syuntaro.

In: Phytochemistry, Vol. 69, No. 1, 01.01.2008, p. 147-153.

Research output: Contribution to journalArticle

Morita, Akio ; Yanagisawa, Osamu ; Takatsu, Satoshi ; Maeda, Setsuko ; Hiradate, Syuntaro. / Mechanism for the detoxification of aluminum in roots of tea plant (Camellia sinensis (L.) Kuntze). In: Phytochemistry. 2008 ; Vol. 69, No. 1. pp. 147-153.
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