Mechanism of hydrolysis of triolein with lipase in a hollow fiber membrane-bioreactor

Masahiro Goto, F. Nakashio, K. Yoshizuka, K. Inoue, Y. Baba

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

The rate of hydrolysis of triolein with lipase in a Teflon hollow fiber membrane bioreactor was quantitatively studied, taking into account the mass transfer of the triolein substrate and the oleic acid reaction product through the membrane. The oleic acid inhibited the hydrolysis at high concentrations, but another reaction product, glycerin, did not inhibit the reaction at all. The hydrolysis of triolein could be explained in terms of a Michaelis-Menten mechanism which takes into account interfacial adsorption and inhibition of the oleic acid. The experimental results could be quantitatively explained by taking simultaneous account of the rate of hydrolysis at the interface and of the rate of mass transfer of triolein and oleic acid to the interface.

Original languageEnglish
Pages (from-to)410-416
Number of pages7
JournalInternational chemical engineering
Volume34
Issue number3
Publication statusPublished - Jul 1 1994

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Oleic acid
Lipases
Bioreactors
Hydrolysis
Membranes
Fibers
Reaction products
Mass transfer
Glycerol
Polytetrafluoroethylenes
Adsorption
Substrates

All Science Journal Classification (ASJC) codes

  • Engineering(all)

Cite this

Mechanism of hydrolysis of triolein with lipase in a hollow fiber membrane-bioreactor. / Goto, Masahiro; Nakashio, F.; Yoshizuka, K.; Inoue, K.; Baba, Y.

In: International chemical engineering, Vol. 34, No. 3, 01.07.1994, p. 410-416.

Research output: Contribution to journalArticle

Goto, M, Nakashio, F, Yoshizuka, K, Inoue, K & Baba, Y 1994, 'Mechanism of hydrolysis of triolein with lipase in a hollow fiber membrane-bioreactor', International chemical engineering, vol. 34, no. 3, pp. 410-416.
Goto, Masahiro ; Nakashio, F. ; Yoshizuka, K. ; Inoue, K. ; Baba, Y. / Mechanism of hydrolysis of triolein with lipase in a hollow fiber membrane-bioreactor. In: International chemical engineering. 1994 ; Vol. 34, No. 3. pp. 410-416.
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