Mechanism of improved cardiac function after bone marrow mononuclear cell therapy: Role of cardiovascular lineage commitment

Chang Hwan Yoon, Masamichi Koyanagi, Kazuma Iekushi, Florian Seeger, Carmen Urbich, Andreas M. Zeiher, Stefanie Dimmeler

Research output: Contribution to journalArticle

97 Citations (Scopus)

Abstract

Background: Cell therapy is a promising option to improve functional recovery after ischemia. Several subsets of bone marrow-derived cells were shown to reduce infarct size and increase ejection fraction in experimental models of ischemia. The mechanisms underlying the functional improvement are diverse and have been shown to include paracrine effects of the injected cells, as well as a variable degree of differentiation to endothelial cells, pericytes, smooth muscle, and cardiac muscle. Methods and Results: To elucidate the true nature of such plasticity and contribution to recovery, we engineered vectors that encoded inducible suicide genes under the control of endothelium (endothelial nitric oxide synthase)-, smooth muscle (SM22α)-, and cardiomyocyte (α-MHC)-specific promoters, thereby allowing selective depletion of the individual cell lineage acquired by the transplanted undifferentiated bone marrow-derived cells. Lentivirally delivered thymidine kinase, which converts the prodrug ganciclovir into a cytotoxic agent, was used to selectively eliminate cells 2 weeks after transplantation of bone marrow mononuclear cells in an acute myocardial infarction model. We demonstrate that elimination of transplanted endothelium-committed or SM22α-expressing cells, but not cardiac-committed cells, induced a significant deterioration of ejection fraction. Moreover, elimination of endothelial nitric oxide synthase-expressing cells 2 weeks after injection reduced capillary and arteriole density. Conclusions: This study demonstrates that elimination of bone marrow mononuclear cells reexpressing endothelial nitric oxide synthase particularly induced a deterioration of cardiac function, which indicates a functional contribution of the vascular cell fate decision of human bone marrow-derived mononuclear cells in vivo.

Original languageEnglish
Pages (from-to)2001-2011
Number of pages11
JournalCirculation
Volume121
Issue number18
DOIs
Publication statusPublished - May 11 2010

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Cell- and Tissue-Based Therapy
Bone Marrow Cells
Nitric Oxide Synthase Type III
Endothelium
Smooth Muscle
Ischemia
Pericytes
Ganciclovir
Thymidine Kinase
Cytotoxins
Prodrugs
Arterioles
Cell Lineage
Bone Marrow Transplantation
Cardiac Myocytes
Suicide
Blood Vessels
Myocardium
Theoretical Models
Endothelial Cells

All Science Journal Classification (ASJC) codes

  • Cardiology and Cardiovascular Medicine
  • Physiology (medical)

Cite this

Yoon, C. H., Koyanagi, M., Iekushi, K., Seeger, F., Urbich, C., Zeiher, A. M., & Dimmeler, S. (2010). Mechanism of improved cardiac function after bone marrow mononuclear cell therapy: Role of cardiovascular lineage commitment. Circulation, 121(18), 2001-2011. https://doi.org/10.1161/CIRCULATIONAHA.109.909291

Mechanism of improved cardiac function after bone marrow mononuclear cell therapy : Role of cardiovascular lineage commitment. / Yoon, Chang Hwan; Koyanagi, Masamichi; Iekushi, Kazuma; Seeger, Florian; Urbich, Carmen; Zeiher, Andreas M.; Dimmeler, Stefanie.

In: Circulation, Vol. 121, No. 18, 11.05.2010, p. 2001-2011.

Research output: Contribution to journalArticle

Yoon, CH, Koyanagi, M, Iekushi, K, Seeger, F, Urbich, C, Zeiher, AM & Dimmeler, S 2010, 'Mechanism of improved cardiac function after bone marrow mononuclear cell therapy: Role of cardiovascular lineage commitment', Circulation, vol. 121, no. 18, pp. 2001-2011. https://doi.org/10.1161/CIRCULATIONAHA.109.909291
Yoon, Chang Hwan ; Koyanagi, Masamichi ; Iekushi, Kazuma ; Seeger, Florian ; Urbich, Carmen ; Zeiher, Andreas M. ; Dimmeler, Stefanie. / Mechanism of improved cardiac function after bone marrow mononuclear cell therapy : Role of cardiovascular lineage commitment. In: Circulation. 2010 ; Vol. 121, No. 18. pp. 2001-2011.
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