Mechanism of murine Vγ1+ γδ T Cell-mediated innate immune response against Listeria monocytogenes infection

Goro Matsuzaki, Hisakata Yamada, Kenji Kishihara, Yasunobu Yoshikai, Kikuo Nomoto

    Research output: Contribution to journalArticle

    34 Citations (Scopus)

    Abstract

    Murine γδ T cells participate in innate immune response against infection of the intracellular bacterium Listeria monocytogenes. In the present report, we analyzed the mechanism of the γδ T cell-mediated response against L. monocytogenes infection. γδ T cell-enriched spleen cells of L. monocytogenes-infected mice produced IFN-γ in vitro in response to L. monocytogenes-infected spleen cells. The IFN-γ production was abrogated by depletion of Vγ1+ γδ T cells. IFN-γ production of the Vγ1+ γδ T cells in response to L. monocytogenes-infected spleen cells required IL-12. However, addition of Fab fragment of anti-TCR γδ monoclonal antibodies (mAb) failed to block the response, suggesting that the response requires no TCR-mediated antigen recognition. Interestingly, Vγ1+ γδ T cells of naive mice also produced IFN-γ in response to L. monocytogenes-infected spleen cells in an IL-12-dependent manner. Furthermore, the IL-12 receptor (IL-12R) gene was expressed on the Vγ1+ γδ T cells of naive mice as well as those of L. monocytogenes-infected mice although naive αβ T cells lack IL-12R expression. All the results suggest that the Vγ1+ γδ T cells participate in immune surveillance against intracellular bacterial infection through quick production of IFN-γ in response to infection-induced IL-12 without antigen-driven clonal expansion and maturation.

    Original languageEnglish
    Pages (from-to)928-935
    Number of pages8
    JournalEuropean Journal of Immunology
    Volume32
    Issue number4
    DOIs
    Publication statusPublished - May 4 2002

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    Listeriosis
    Listeria monocytogenes
    Innate Immunity
    T-Lymphocytes
    Interleukin-12 Receptors
    Spleen
    Interleukin-12
    Antigens
    Immunoglobulin Fab Fragments
    Infection
    Bacterial Infections
    Monoclonal Antibodies
    Bacteria

    All Science Journal Classification (ASJC) codes

    • Immunology and Allergy
    • Immunology

    Cite this

    Mechanism of murine Vγ1+ γδ T Cell-mediated innate immune response against Listeria monocytogenes infection. / Matsuzaki, Goro; Yamada, Hisakata; Kishihara, Kenji; Yoshikai, Yasunobu; Nomoto, Kikuo.

    In: European Journal of Immunology, Vol. 32, No. 4, 04.05.2002, p. 928-935.

    Research output: Contribution to journalArticle

    Matsuzaki, Goro ; Yamada, Hisakata ; Kishihara, Kenji ; Yoshikai, Yasunobu ; Nomoto, Kikuo. / Mechanism of murine Vγ1+ γδ T Cell-mediated innate immune response against Listeria monocytogenes infection. In: European Journal of Immunology. 2002 ; Vol. 32, No. 4. pp. 928-935.
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