Mechanism of the cytolytic action of Pseudomonas aeruginosa cytotoxin: Oligomerization of the cytotoxin on target membranes

Makoto Ohnishi, Tetsuya Hayashi, Toshio Tomita, Yoshiro Terawaki

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Abstract

Pseudomonas aeruginosa cytotoxin (CTX) is thought to be a pore-forming polypeptide of 29 kDa. To study whether CTX assembles into oligomer on target membranes, we solubilized membrane-bound toxin with 1% sodium dodecyl sulfate (SDS) at 25°C and analyzed its molecular size using SDS-polyacrylamide gel electrophoresis and immunoblot analysis. The results indicate that CTX forms a complex of approximately 145 kDa on the surface of erythrocytes and lipid vesicles, and that the complex formation is closely correlated with the toxin-induced permeabilization of target membranes. Thus, CTX may assemble into a pore-forming oligomer on target membranes.

Original languageEnglish
Pages (from-to)357-360
Number of pages4
JournalFEBS Letters
Volume356
Issue number2-3
DOIs
Publication statusPublished - Dec 19 1994
Externally publishedYes

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All Science Journal Classification (ASJC) codes

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology

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