Mechanisms and consequences of carnosine-induced activation of intestinal epithelial cells

Keishi Kadooka, Kaoru Fujii, Takashi Matsumoto, Mikako Sato, Fumiki Morimatsu, Kosuke Tashiro, Satoru Kuhara, Yoshinori Katakura

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

The molecular basis for the carnosine-induced activation of intestinal epithelial cells was studied and subsequently we focused on whether carnosine stimulates a brain-gut interaction. To assess this, we investigated changes in intestinal epithelial cells induced by carnosine. Our results showed that carnosine activated Caco-2 cells, resulting in the secretion of various factors (including neurotrophic factors), and leading to the induction of neurite growth in SY-SY5Y cells. We then conducted DNA microarray analysis to reveal global changes in Caco-2 cells via treatment with carnosine. The expression of 745 genes significantly changed upon carnosine treatment. Furthermore, cluster analysis showed that several of these genes were related to secretory proteins, membrane protein/transporters, and calcium channel/transport protein. Some of these genes would explain the mechanism of carnosine action, especially considering stimulation of the brain-gut interaction.

Original languageEnglish
Pages (from-to)32-37
Number of pages6
JournalJournal of Functional Foods
Volume13
DOIs
Publication statusPublished - Jan 1 2015

Fingerprint

Carnosine
carnosine
epithelial cells
Epithelial Cells
Caco-2 Cells
digestive system
brain
neurotrophins
neurites
calcium channels
Membrane Transport Proteins
Nerve Growth Factors
transport proteins
Brain
Neurites
cells
Calcium Channels
Microarray Analysis
global change
Oligonucleotide Array Sequence Analysis

All Science Journal Classification (ASJC) codes

  • Food Science
  • Medicine (miscellaneous)
  • Nutrition and Dietetics

Cite this

Mechanisms and consequences of carnosine-induced activation of intestinal epithelial cells. / Kadooka, Keishi; Fujii, Kaoru; Matsumoto, Takashi; Sato, Mikako; Morimatsu, Fumiki; Tashiro, Kosuke; Kuhara, Satoru; Katakura, Yoshinori.

In: Journal of Functional Foods, Vol. 13, 01.01.2015, p. 32-37.

Research output: Contribution to journalArticle

Kadooka, Keishi ; Fujii, Kaoru ; Matsumoto, Takashi ; Sato, Mikako ; Morimatsu, Fumiki ; Tashiro, Kosuke ; Kuhara, Satoru ; Katakura, Yoshinori. / Mechanisms and consequences of carnosine-induced activation of intestinal epithelial cells. In: Journal of Functional Foods. 2015 ; Vol. 13. pp. 32-37.
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