TY - JOUR
T1 - Mechanisms and consequences of carnosine-induced activation of intestinal epithelial cells
AU - Kadooka, Keishi
AU - Fujii, Kaoru
AU - Matsumoto, Takashi
AU - Sato, Mikako
AU - Morimatsu, Fumiki
AU - Tashiro, Kosuke
AU - Kuhara, Satoru
AU - Katakura, Yoshinori
N1 - Funding Information:
The authors like to thank N. Oshima (GE Healthcare) for her expert assistance with the IN Cell Analyzer 1000, Dr. M. Udono and Mr. M. Deguchi for their technical assistance, and Dr. K. Yasuda for her expert assistance with DNA microarray analysis. This work was supported by JSPS KAKENHI Grant Number 12J02731 .
Publisher Copyright:
© 2014 Elsevier Ltd.
PY - 2015/3/1
Y1 - 2015/3/1
N2 - The molecular basis for the carnosine-induced activation of intestinal epithelial cells was studied and subsequently we focused on whether carnosine stimulates a brain-gut interaction. To assess this, we investigated changes in intestinal epithelial cells induced by carnosine. Our results showed that carnosine activated Caco-2 cells, resulting in the secretion of various factors (including neurotrophic factors), and leading to the induction of neurite growth in SY-SY5Y cells. We then conducted DNA microarray analysis to reveal global changes in Caco-2 cells via treatment with carnosine. The expression of 745 genes significantly changed upon carnosine treatment. Furthermore, cluster analysis showed that several of these genes were related to secretory proteins, membrane protein/transporters, and calcium channel/transport protein. Some of these genes would explain the mechanism of carnosine action, especially considering stimulation of the brain-gut interaction.
AB - The molecular basis for the carnosine-induced activation of intestinal epithelial cells was studied and subsequently we focused on whether carnosine stimulates a brain-gut interaction. To assess this, we investigated changes in intestinal epithelial cells induced by carnosine. Our results showed that carnosine activated Caco-2 cells, resulting in the secretion of various factors (including neurotrophic factors), and leading to the induction of neurite growth in SY-SY5Y cells. We then conducted DNA microarray analysis to reveal global changes in Caco-2 cells via treatment with carnosine. The expression of 745 genes significantly changed upon carnosine treatment. Furthermore, cluster analysis showed that several of these genes were related to secretory proteins, membrane protein/transporters, and calcium channel/transport protein. Some of these genes would explain the mechanism of carnosine action, especially considering stimulation of the brain-gut interaction.
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U2 - 10.1016/j.jff.2014.12.024
DO - 10.1016/j.jff.2014.12.024
M3 - Article
AN - SCOPUS:84922496611
SN - 1756-4646
VL - 13
SP - 32
EP - 37
JO - Journal of Functional Foods
JF - Journal of Functional Foods
ER -