Mechanistic insights into the 1,3-xylanases: Useful enzymes for manipulation of algal biomass

Ethan D. Goddard-Borger, Keishi Sakaguchi, Stephan Reitinger, Nobuhisa Watanabe, Makoto Ito, Stephen G. Withers

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Xylanases capable of degrading the crystalline microfibrils of 1,3-xylan that reinforce the cell walls of some red and siphonous green algae have not been well studied, yet they could prove to be of great utility in algaculture for the production of food and renewable chemical feedstocks. To gain a better mechanistic understanding of these enzymes, a suite of reagents was synthesized and evaluated as substrates and inhibitors of an endo-1,3-xylanase. With these reagents, a retaining mechanism was confirmed for the xylanase, its catalytic nucleophile identified, and the existence of -3 to +2 substrate-binding subsites demonstrated. Protein crystal X-ray diffraction methods provided a high resolution structure of a trapped covalent glycosyl-enzyme intermediate, indicating that the 1,3-xylanases likely utilize the 1S 34H 34C 1 conformational itinerary to effect catalysis.

Original languageEnglish
Pages (from-to)3895-3902
Number of pages8
JournalJournal of the American Chemical Society
Volume134
Issue number8
DOIs
Publication statusPublished - Feb 29 2012

Fingerprint

Biomass
Enzymes
Microfibrils
Nucleophiles
Chlorophyta
Substrates
Algae
Catalysis
X-Ray Diffraction
Cell Wall
Feedstocks
Cells
Crystalline materials
Proteins
Food
X ray diffraction
Crystals
Xylans
1,3-xylan

All Science Journal Classification (ASJC) codes

  • Catalysis
  • Chemistry(all)
  • Biochemistry
  • Colloid and Surface Chemistry

Cite this

Mechanistic insights into the 1,3-xylanases : Useful enzymes for manipulation of algal biomass. / Goddard-Borger, Ethan D.; Sakaguchi, Keishi; Reitinger, Stephan; Watanabe, Nobuhisa; Ito, Makoto; Withers, Stephen G.

In: Journal of the American Chemical Society, Vol. 134, No. 8, 29.02.2012, p. 3895-3902.

Research output: Contribution to journalArticle

Goddard-Borger, Ethan D. ; Sakaguchi, Keishi ; Reitinger, Stephan ; Watanabe, Nobuhisa ; Ito, Makoto ; Withers, Stephen G. / Mechanistic insights into the 1,3-xylanases : Useful enzymes for manipulation of algal biomass. In: Journal of the American Chemical Society. 2012 ; Vol. 134, No. 8. pp. 3895-3902.
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