Mechanistic insights into the activation of Rad51-mediated strand exchange from the structure of a recombination activator, the Swi5-Sfr1 complex

Naoyuki Kuwabara, Yasuto Murayama, Hiroshi Hashimoto, Yuuichi Kokabu, Mitsunori Ikeguchi, Mamoru Sato, Kouta Mayanagi, Yasuhiro Tsutsui, Hiroshi Iwasaki, Toshiyuki Shimizu

    Research output: Contribution to journalArticle

    18 Citations (Scopus)

    Abstract

    Rad51 forms a helical filament on single-stranded DNA and promotes strand exchange between two homologous DNA molecules during homologous recombination. The Swi5-Sfr1 complex interacts directly with Rad51 and stimulates strand exchange. Here we describe structural and functional aspects of the complex. Swi5 and the C-terminal core domain of Sfr1 form an essential activator complex with a parallel coiled-coil heterodimer joined firmly together via two previously uncharacterized leucine-zipper motifs and a bundle. The resultant coiled coil is sharply kinked, generating an elongated crescent-shaped structure suitable for transient binding within the helical groove of the Rad51 filament. The N-terminal region of Sfr1, meanwhile, has an interface for binding of Rad51. Our data suggest that the snug fit resulting from the complementary geometry of the heterodimer activates the Rad51 filament and that the N-terminal domain of Sfr1 plays a role in the efficient recruitment of the Swi5-Sfr1 complex to the Rad51 filaments.

    Original languageEnglish
    Pages (from-to)440-449
    Number of pages10
    JournalStructure
    Volume20
    Issue number3
    DOIs
    Publication statusPublished - Mar 7 2012

    Fingerprint

    Leucine Zippers
    Single-Stranded DNA
    Homologous Recombination
    Genetic Recombination
    DNA

    All Science Journal Classification (ASJC) codes

    • Structural Biology
    • Molecular Biology

    Cite this

    Mechanistic insights into the activation of Rad51-mediated strand exchange from the structure of a recombination activator, the Swi5-Sfr1 complex. / Kuwabara, Naoyuki; Murayama, Yasuto; Hashimoto, Hiroshi; Kokabu, Yuuichi; Ikeguchi, Mitsunori; Sato, Mamoru; Mayanagi, Kouta; Tsutsui, Yasuhiro; Iwasaki, Hiroshi; Shimizu, Toshiyuki.

    In: Structure, Vol. 20, No. 3, 07.03.2012, p. 440-449.

    Research output: Contribution to journalArticle

    Kuwabara, N, Murayama, Y, Hashimoto, H, Kokabu, Y, Ikeguchi, M, Sato, M, Mayanagi, K, Tsutsui, Y, Iwasaki, H & Shimizu, T 2012, 'Mechanistic insights into the activation of Rad51-mediated strand exchange from the structure of a recombination activator, the Swi5-Sfr1 complex', Structure, vol. 20, no. 3, pp. 440-449. https://doi.org/10.1016/j.str.2012.01.005
    Kuwabara, Naoyuki ; Murayama, Yasuto ; Hashimoto, Hiroshi ; Kokabu, Yuuichi ; Ikeguchi, Mitsunori ; Sato, Mamoru ; Mayanagi, Kouta ; Tsutsui, Yasuhiro ; Iwasaki, Hiroshi ; Shimizu, Toshiyuki. / Mechanistic insights into the activation of Rad51-mediated strand exchange from the structure of a recombination activator, the Swi5-Sfr1 complex. In: Structure. 2012 ; Vol. 20, No. 3. pp. 440-449.
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