Medroxyprogesterone acetate inhibits proliferation of colon cancer cell lines by modulating cell cycle-related protein expression

Yoshihiro Tanaka, Kiyoko Kato, Ryuichi Mibu, Souko Uchida, Kazuo Asanoma, Kazunori Hashimoto, Masahiro Nozaki, Norio Wake

Research output: Contribution to journalArticlepeer-review

17 Citations (Scopus)

Abstract

OBJECTIVE: To investigate the effects of medroxyprogesterone acetate on colon cancer cells in vitro. DESIGN: HT29 and HCT116 human colon cancer cell lines were used in this study. Cell growth and WST-1 assays were performed to investigate the antiproliferative effect of medroxyprogesterone acetate. Cell cycle analysis was performed to investigate the effects of medroxyprogesterone acetate on cell cycle distribution. Western blot, immunoprecipitation, and a cyclin-dependent kinase assay were performed to investigate changes in the levels of cell cycle proteins. RESULTS: Medroxyprogesterone acetate inhibited proliferation of the cancer cells by inducing accumulation in the G0/G1 fraction. Medroxyprogesterone acetate decreased expression of cyclin E, increased expression of p21, and enhanced interaction of p21 with cyclin-dependent kinase 2, eventually inhibiting its activity. CONCLUSIONS: Medroxyprogesterone acetate exerts its antiproliferative effect by modulating cell cycle-related protein expression and cyclin-dependent kinase 2 activity. These results should help to elucidate the protective effect of medroxyprogesterone acetate on colon cancer risk.

Original languageEnglish
Pages (from-to)442-453
Number of pages12
JournalMenopause
Volume15
Issue number3
DOIs
Publication statusPublished - May 2008

All Science Journal Classification (ASJC) codes

  • Obstetrics and Gynaecology

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