Meiotic and epigenetic aberrations in Dmnt3L-deficient male germ cells

Kenichiro Hata, Maki Kusumi, Takaaki Yokomine, En Li, Hiroyuki Sasaki

Research output: Contribution to journalArticle

72 Citations (Scopus)

Abstract

The DNA methyltransferase-like protein Dnmt3L is necessary for the establishment of genomic imprints in oogenesis and for normal spermatogenesis (Bourc'his et al., 2001; Hata et al., 2002). Also, a paternally imprinted gene, H19, loses DNA methylation in Dnmt3L-/- spermatogonia (Bourc'his and Bestor, 2004; Kaneda et al., 2004). To determine the reason for the impaired spermatogenesis in the Dnmt3L-/- testes, we have carried out a series of histological and molecular studies. We show here that Dnmt3L-/- germ cells were arrested and died around the early meiotic stage. A microarray-based gene expression-profiling analysis revealed that various gonad-specific and/or sex-chromosome-linked genes were downregulated in the Dnmt3L-/-testes. In contrast, expression of retrovirus-like intracisternal A-particle (IAP) sequences was upregulated; consistent with this observation, a specific IAP copy showed complete loss of DNA methylation. These findings indicate that Dnmt3L regulates germ cell-specific gene expression and IAP suppression, which are critical for male germ cell proliferation and meiosis.

Original languageEnglish
Pages (from-to)116-122
Number of pages7
JournalMolecular Reproduction and Development
Volume73
Issue number1
DOIs
Publication statusPublished - Jan 1 2006
Externally publishedYes

Fingerprint

Epigenomics
Germ Cells
DNA Methylation
Spermatogenesis
Testis
Protein Methyltransferases
Oogenesis
Spermatogonia
Sex Chromosomes
Meiosis
Gonads
Gene Expression Profiling
Retroviridae
Genes
Down-Regulation
Cell Proliferation
Gene Expression
DNA

All Science Journal Classification (ASJC) codes

  • Genetics
  • Developmental Biology
  • Cell Biology

Cite this

Meiotic and epigenetic aberrations in Dmnt3L-deficient male germ cells. / Hata, Kenichiro; Kusumi, Maki; Yokomine, Takaaki; Li, En; Sasaki, Hiroyuki.

In: Molecular Reproduction and Development, Vol. 73, No. 1, 01.01.2006, p. 116-122.

Research output: Contribution to journalArticle

Hata, Kenichiro ; Kusumi, Maki ; Yokomine, Takaaki ; Li, En ; Sasaki, Hiroyuki. / Meiotic and epigenetic aberrations in Dmnt3L-deficient male germ cells. In: Molecular Reproduction and Development. 2006 ; Vol. 73, No. 1. pp. 116-122.
@article{0881614589b64aebb4f0bd7f6bb8fa21,
title = "Meiotic and epigenetic aberrations in Dmnt3L-deficient male germ cells",
abstract = "The DNA methyltransferase-like protein Dnmt3L is necessary for the establishment of genomic imprints in oogenesis and for normal spermatogenesis (Bourc'his et al., 2001; Hata et al., 2002). Also, a paternally imprinted gene, H19, loses DNA methylation in Dnmt3L-/- spermatogonia (Bourc'his and Bestor, 2004; Kaneda et al., 2004). To determine the reason for the impaired spermatogenesis in the Dnmt3L-/- testes, we have carried out a series of histological and molecular studies. We show here that Dnmt3L-/- germ cells were arrested and died around the early meiotic stage. A microarray-based gene expression-profiling analysis revealed that various gonad-specific and/or sex-chromosome-linked genes were downregulated in the Dnmt3L-/-testes. In contrast, expression of retrovirus-like intracisternal A-particle (IAP) sequences was upregulated; consistent with this observation, a specific IAP copy showed complete loss of DNA methylation. These findings indicate that Dnmt3L regulates germ cell-specific gene expression and IAP suppression, which are critical for male germ cell proliferation and meiosis.",
author = "Kenichiro Hata and Maki Kusumi and Takaaki Yokomine and En Li and Hiroyuki Sasaki",
year = "2006",
month = "1",
day = "1",
doi = "10.1002/mrd.20387",
language = "English",
volume = "73",
pages = "116--122",
journal = "Molecular Reproduction and Development",
issn = "1040-452X",
publisher = "Wiley-Liss Inc.",
number = "1",

}

TY - JOUR

T1 - Meiotic and epigenetic aberrations in Dmnt3L-deficient male germ cells

AU - Hata, Kenichiro

AU - Kusumi, Maki

AU - Yokomine, Takaaki

AU - Li, En

AU - Sasaki, Hiroyuki

PY - 2006/1/1

Y1 - 2006/1/1

N2 - The DNA methyltransferase-like protein Dnmt3L is necessary for the establishment of genomic imprints in oogenesis and for normal spermatogenesis (Bourc'his et al., 2001; Hata et al., 2002). Also, a paternally imprinted gene, H19, loses DNA methylation in Dnmt3L-/- spermatogonia (Bourc'his and Bestor, 2004; Kaneda et al., 2004). To determine the reason for the impaired spermatogenesis in the Dnmt3L-/- testes, we have carried out a series of histological and molecular studies. We show here that Dnmt3L-/- germ cells were arrested and died around the early meiotic stage. A microarray-based gene expression-profiling analysis revealed that various gonad-specific and/or sex-chromosome-linked genes were downregulated in the Dnmt3L-/-testes. In contrast, expression of retrovirus-like intracisternal A-particle (IAP) sequences was upregulated; consistent with this observation, a specific IAP copy showed complete loss of DNA methylation. These findings indicate that Dnmt3L regulates germ cell-specific gene expression and IAP suppression, which are critical for male germ cell proliferation and meiosis.

AB - The DNA methyltransferase-like protein Dnmt3L is necessary for the establishment of genomic imprints in oogenesis and for normal spermatogenesis (Bourc'his et al., 2001; Hata et al., 2002). Also, a paternally imprinted gene, H19, loses DNA methylation in Dnmt3L-/- spermatogonia (Bourc'his and Bestor, 2004; Kaneda et al., 2004). To determine the reason for the impaired spermatogenesis in the Dnmt3L-/- testes, we have carried out a series of histological and molecular studies. We show here that Dnmt3L-/- germ cells were arrested and died around the early meiotic stage. A microarray-based gene expression-profiling analysis revealed that various gonad-specific and/or sex-chromosome-linked genes were downregulated in the Dnmt3L-/-testes. In contrast, expression of retrovirus-like intracisternal A-particle (IAP) sequences was upregulated; consistent with this observation, a specific IAP copy showed complete loss of DNA methylation. These findings indicate that Dnmt3L regulates germ cell-specific gene expression and IAP suppression, which are critical for male germ cell proliferation and meiosis.

UR - http://www.scopus.com/inward/record.url?scp=28744442731&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=28744442731&partnerID=8YFLogxK

U2 - 10.1002/mrd.20387

DO - 10.1002/mrd.20387

M3 - Article

C2 - 16211598

AN - SCOPUS:28744442731

VL - 73

SP - 116

EP - 122

JO - Molecular Reproduction and Development

JF - Molecular Reproduction and Development

SN - 1040-452X

IS - 1

ER -