Meiotic cell cycle in Xenopus oocytes is independent of cdk2 kinase

Nobuaki Furuno, Yasuki Ogawa, Jun Iwashita, Nobushige Nakajo, Noriyuki Sagata

Research output: Contribution to journalArticle

38 Citations (Scopus)

Abstract

In vertebrates, M phase-promoting factor (MPF), a universal G2/M regulator in eukaryotic cells, drives meiotic maturation of oocytes, while cytostatic factor (CSF) arrests mature oocytes at metaphase II until fertilization. Cdk2 kinase, a G1/S regulator in higher eukaryotic cells, is activated during meiotic maturation of Xenopus oocytes and, like Mos (an essential component of CSF), is proposed to be involved in metaphase II arrest in mature oocytes. In addition, cdk2 kinase has been shown recently to be essential for MPF activation in Xenopus embryonic mitosis. Here we report injection of Xenopus oocytes with the cdk2 kinase inhibitor p21(Cip) in order to (re)evaluate the role of cdk2 kinase in oocyte meiosis. Immature oocytes injected with p21(Cip) can enter both meiosis I and meiosis II normally, as evidenced by the typical fluctuations in MPF activity. Moreover, mature oocytes injected with p21(Cip) are retained normally in metaphase II for a prolonged period, whereas those injected with neutralizing anti-Mos antibody are released readily from metaphase II arrest. These results argue strongly against a role for cdk2 kinase in MPF activation and its proposed role in metaphase II arrest, in Xenopus oocyte meiosis. We discuss the possibility that cdk2 kinase stored in oocytes may function, as a maternal protein, solely for early embryonic cell cycles.

Original languageEnglish
Pages (from-to)3860-3865
Number of pages6
JournalEMBO Journal
Volume16
Issue number13
DOIs
Publication statusPublished - Jul 1 1997

Fingerprint

Xenopus
Oocytes
Cell Cycle
Phosphotransferases
Maturation-Promoting Factor
Cells
Metaphase
Meiosis
Proto-Oncogene Proteins c-mos
Eukaryotic Cells
Chemical activation
Neutralizing Antibodies
Mitosis
Fertilization
Vertebrates
Anti-Idiotypic Antibodies
Mothers
Antibodies
Injections

All Science Journal Classification (ASJC) codes

  • Neuroscience(all)
  • Molecular Biology
  • Biochemistry, Genetics and Molecular Biology(all)
  • Immunology and Microbiology(all)

Cite this

Meiotic cell cycle in Xenopus oocytes is independent of cdk2 kinase. / Furuno, Nobuaki; Ogawa, Yasuki; Iwashita, Jun; Nakajo, Nobushige; Sagata, Noriyuki.

In: EMBO Journal, Vol. 16, No. 13, 01.07.1997, p. 3860-3865.

Research output: Contribution to journalArticle

Furuno, N, Ogawa, Y, Iwashita, J, Nakajo, N & Sagata, N 1997, 'Meiotic cell cycle in Xenopus oocytes is independent of cdk2 kinase', EMBO Journal, vol. 16, no. 13, pp. 3860-3865. https://doi.org/10.1093/emboj/16.13.3860
Furuno, Nobuaki ; Ogawa, Yasuki ; Iwashita, Jun ; Nakajo, Nobushige ; Sagata, Noriyuki. / Meiotic cell cycle in Xenopus oocytes is independent of cdk2 kinase. In: EMBO Journal. 1997 ; Vol. 16, No. 13. pp. 3860-3865.
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