Melatonin-induced changes in the expression of thyroid hormone-converting enzymes in hypothalamus depend on the timing of melatonin injections and genetic background in mice

Mariko Goto, Haruka Matsuo, Masayuki Iigo, Mitsuhiro Furuse, Horst Werner Korf, Shinobu Yasuo

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4 Citations (Scopus)

Abstract

Recent studies have identified TSHB, Dio2, and Dio3 as key genes for the photoperiodic regulation of gonads. In mammals, the expression of these genes is controlled by melatonin. Surprisingly, this effect of melatonin was shown to be conserved in several reproductively non-photoperiodic laboratory mouse strains that have thus become a valuable model to decipher the mechanisms through which melatonin controls the expression of TSHB, Dio2, and Dio3. In this study, we assessed the effects of intraperitoneal melatonin injections and of their timing on the expression of TSHB, TSHR, Dio2, and Dio3 in the hypothalamo-hypophysial systems of melatonin-proficient CBA/N and melatonin-deficient C57BL/6J mice kept under long-day conditions. In CBA/N mice, Dio3 expression was induced by a daily melatonin injection at ZT14 only, whereas in C57BL/6J mice, a daily melatonin injection induced Dio3 expression at all time points investigated (ZT8, 14, and 20) without changes in TSHB expression in both strains. Dio2 expression was suppressed by a daily melatonin injection only in C57BL/6J mice and only at ZT8. Effect of a daily melatonin injection on TSHR expression was strain- and region- specific. Melatonin levels elevated in plasma and hypothalamus after intraperitoneal injections of melatonin at ZT8 for 7. days in C57BL/6J returned to basal levels within 1. h after the final injection, while in CBA/N mice melatonin levels in hypothalamus remained high for at least 1. h. These data suggest that Dio2 and Dio3 expression in the hypothalamus is differentially regulated by the timing of melatonin injections through strain-specific mechanisms.

Original languageEnglish
Pages (from-to)33-40
Number of pages8
JournalGeneral and Comparative Endocrinology
Volume186
DOIs
Publication statusPublished - Jun 1 2013

Fingerprint

melatonin
Melatonin
thyroid hormones
hypothalamus
Thyroid Hormones
genetic background
Hypothalamus
injection
Injections
mice
Enzymes
enzymes
Inbred C57BL Mouse
Inbred CBA Mouse
Genetic Background
Intraperitoneal Injections
Hypothalamo-Hypophyseal System
Gonads
intraperitoneal injection
gonads

All Science Journal Classification (ASJC) codes

  • Animal Science and Zoology
  • Endocrinology

Cite this

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title = "Melatonin-induced changes in the expression of thyroid hormone-converting enzymes in hypothalamus depend on the timing of melatonin injections and genetic background in mice",
abstract = "Recent studies have identified TSHB, Dio2, and Dio3 as key genes for the photoperiodic regulation of gonads. In mammals, the expression of these genes is controlled by melatonin. Surprisingly, this effect of melatonin was shown to be conserved in several reproductively non-photoperiodic laboratory mouse strains that have thus become a valuable model to decipher the mechanisms through which melatonin controls the expression of TSHB, Dio2, and Dio3. In this study, we assessed the effects of intraperitoneal melatonin injections and of their timing on the expression of TSHB, TSHR, Dio2, and Dio3 in the hypothalamo-hypophysial systems of melatonin-proficient CBA/N and melatonin-deficient C57BL/6J mice kept under long-day conditions. In CBA/N mice, Dio3 expression was induced by a daily melatonin injection at ZT14 only, whereas in C57BL/6J mice, a daily melatonin injection induced Dio3 expression at all time points investigated (ZT8, 14, and 20) without changes in TSHB expression in both strains. Dio2 expression was suppressed by a daily melatonin injection only in C57BL/6J mice and only at ZT8. Effect of a daily melatonin injection on TSHR expression was strain- and region- specific. Melatonin levels elevated in plasma and hypothalamus after intraperitoneal injections of melatonin at ZT8 for 7. days in C57BL/6J returned to basal levels within 1. h after the final injection, while in CBA/N mice melatonin levels in hypothalamus remained high for at least 1. h. These data suggest that Dio2 and Dio3 expression in the hypothalamus is differentially regulated by the timing of melatonin injections through strain-specific mechanisms.",
author = "Mariko Goto and Haruka Matsuo and Masayuki Iigo and Mitsuhiro Furuse and Korf, {Horst Werner} and Shinobu Yasuo",
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T1 - Melatonin-induced changes in the expression of thyroid hormone-converting enzymes in hypothalamus depend on the timing of melatonin injections and genetic background in mice

AU - Goto, Mariko

AU - Matsuo, Haruka

AU - Iigo, Masayuki

AU - Furuse, Mitsuhiro

AU - Korf, Horst Werner

AU - Yasuo, Shinobu

PY - 2013/6/1

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N2 - Recent studies have identified TSHB, Dio2, and Dio3 as key genes for the photoperiodic regulation of gonads. In mammals, the expression of these genes is controlled by melatonin. Surprisingly, this effect of melatonin was shown to be conserved in several reproductively non-photoperiodic laboratory mouse strains that have thus become a valuable model to decipher the mechanisms through which melatonin controls the expression of TSHB, Dio2, and Dio3. In this study, we assessed the effects of intraperitoneal melatonin injections and of their timing on the expression of TSHB, TSHR, Dio2, and Dio3 in the hypothalamo-hypophysial systems of melatonin-proficient CBA/N and melatonin-deficient C57BL/6J mice kept under long-day conditions. In CBA/N mice, Dio3 expression was induced by a daily melatonin injection at ZT14 only, whereas in C57BL/6J mice, a daily melatonin injection induced Dio3 expression at all time points investigated (ZT8, 14, and 20) without changes in TSHB expression in both strains. Dio2 expression was suppressed by a daily melatonin injection only in C57BL/6J mice and only at ZT8. Effect of a daily melatonin injection on TSHR expression was strain- and region- specific. Melatonin levels elevated in plasma and hypothalamus after intraperitoneal injections of melatonin at ZT8 for 7. days in C57BL/6J returned to basal levels within 1. h after the final injection, while in CBA/N mice melatonin levels in hypothalamus remained high for at least 1. h. These data suggest that Dio2 and Dio3 expression in the hypothalamus is differentially regulated by the timing of melatonin injections through strain-specific mechanisms.

AB - Recent studies have identified TSHB, Dio2, and Dio3 as key genes for the photoperiodic regulation of gonads. In mammals, the expression of these genes is controlled by melatonin. Surprisingly, this effect of melatonin was shown to be conserved in several reproductively non-photoperiodic laboratory mouse strains that have thus become a valuable model to decipher the mechanisms through which melatonin controls the expression of TSHB, Dio2, and Dio3. In this study, we assessed the effects of intraperitoneal melatonin injections and of their timing on the expression of TSHB, TSHR, Dio2, and Dio3 in the hypothalamo-hypophysial systems of melatonin-proficient CBA/N and melatonin-deficient C57BL/6J mice kept under long-day conditions. In CBA/N mice, Dio3 expression was induced by a daily melatonin injection at ZT14 only, whereas in C57BL/6J mice, a daily melatonin injection induced Dio3 expression at all time points investigated (ZT8, 14, and 20) without changes in TSHB expression in both strains. Dio2 expression was suppressed by a daily melatonin injection only in C57BL/6J mice and only at ZT8. Effect of a daily melatonin injection on TSHR expression was strain- and region- specific. Melatonin levels elevated in plasma and hypothalamus after intraperitoneal injections of melatonin at ZT8 for 7. days in C57BL/6J returned to basal levels within 1. h after the final injection, while in CBA/N mice melatonin levels in hypothalamus remained high for at least 1. h. These data suggest that Dio2 and Dio3 expression in the hypothalamus is differentially regulated by the timing of melatonin injections through strain-specific mechanisms.

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