Membrane nanotube formation in osteoclastogenesis

Toshio Kukita, akira takahashi, Jing Qi Zhang, Akiko Kukita

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Membrane tunneling nanotubes (TNTs) are unique intercellular structures, which enable rapid transport of various materials and rapid communication between cells present in a long distance. During osteoclas-togenesis, mononuclear osteoclast precursors form abundant TNTs in prior to cell–cell fusion. Here we introduce a protocol for detecting TNTs during osteoclastogenesis by use of live cell imaging utilizing a confocal laser microscopy. We also demonstrate a standard protocol for observation of TNTs by scanning electron microscope.

Original languageEnglish
Pages (from-to)193-202
Number of pages10
JournalMethods in Molecular Biology
Volume1313
DOIs
Publication statusPublished - Jan 1 2015

Fingerprint

Nanotubes
Osteogenesis
Membranes
Confocal Microscopy
Osteoclasts
Cell Communication
Observation
Electrons

All Science Journal Classification (ASJC) codes

  • Molecular Biology
  • Genetics

Cite this

Membrane nanotube formation in osteoclastogenesis. / Kukita, Toshio; takahashi, akira; Zhang, Jing Qi; Kukita, Akiko.

In: Methods in Molecular Biology, Vol. 1313, 01.01.2015, p. 193-202.

Research output: Contribution to journalArticle

Kukita, Toshio ; takahashi, akira ; Zhang, Jing Qi ; Kukita, Akiko. / Membrane nanotube formation in osteoclastogenesis. In: Methods in Molecular Biology. 2015 ; Vol. 1313. pp. 193-202.
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