Membrane nanotube formation in osteoclastogenesis

Toshio Kukita; Akira Takahashi; Jing Qi Zhang; Akiko Kukita

doi:10.1007/978-1-4939-2703-6_14

Membrane nanotube formation in osteoclastogenesis

Toshio Kukita, Akira Takahashi, Jing Qi Zhang, Akiko Kukita

Research output: Chapter in Book/Report/Conference proceeding › Chapter

Abstract

Membrane tunneling nanotubes (TNT_s) are unique intercellular structures, which enable rapid transport of various materials and rapid communication between cells present in a long distance. During osteoclastogenesis, mononuclear osteoclast precursors form abundant TNT_s in prior to cell-cell fusion. Here we introduce a protocol for detecting TNTs during osteoclastogenesis by use of live cell imaging utilizing a confocal laser microscopy. We also demonstrate a standard protocol for observation of TNTs by scanning electron microscope.

Original language	English
Title of host publication	Cell Fusion
Subtitle of host publication	Overviews and Methods: Second Edition
Publisher	Springer New York
Pages	193-202
Number of pages	10
ISBN (Electronic)	9781493927036
ISBN (Print)	9781493927029
DOIs	https://doi.org/10.1007/978-1-4939-2703-6_14
Publication status	Published - May 6 2015

All Science Journal Classification (ASJC) codes

Biochemistry, Genetics and Molecular Biology(all)

Access to Document

10.1007/978-1-4939-2703-6_14

Fingerprint Dive into the research topics of 'Membrane nanotube formation in osteoclastogenesis'. Together they form a unique fingerprint.

Cite this

@inbook{539001f8b8a244148d204883cf263cf5,

title = "Membrane nanotube formation in osteoclastogenesis",

abstract = "Membrane tunneling nanotubes (TNTs) are unique intercellular structures, which enable rapid transport of various materials and rapid communication between cells present in a long distance. During osteoclastogenesis, mononuclear osteoclast precursors form abundant TNTs in prior to cell-cell fusion. Here we introduce a protocol for detecting TNTs during osteoclastogenesis by use of live cell imaging utilizing a confocal laser microscopy. We also demonstrate a standard protocol for observation of TNTs by scanning electron microscope.",

author = "Toshio Kukita and Akira Takahashi and Zhang, {Jing Qi} and Akiko Kukita",

year = "2015",

month = may,

day = "6",

doi = "10.1007/978-1-4939-2703-6_14",

language = "English",

isbn = "9781493927029",

pages = "193--202",

booktitle = "Cell Fusion",

publisher = "Springer New York",

address = "United States",

}

TY - CHAP

T1 - Membrane nanotube formation in osteoclastogenesis

AU - Kukita, Toshio

AU - Takahashi, Akira

AU - Zhang, Jing Qi

AU - Kukita, Akiko

PY - 2015/5/6

Y1 - 2015/5/6

N2 - Membrane tunneling nanotubes (TNTs) are unique intercellular structures, which enable rapid transport of various materials and rapid communication between cells present in a long distance. During osteoclastogenesis, mononuclear osteoclast precursors form abundant TNTs in prior to cell-cell fusion. Here we introduce a protocol for detecting TNTs during osteoclastogenesis by use of live cell imaging utilizing a confocal laser microscopy. We also demonstrate a standard protocol for observation of TNTs by scanning electron microscope.

AB - Membrane tunneling nanotubes (TNTs) are unique intercellular structures, which enable rapid transport of various materials and rapid communication between cells present in a long distance. During osteoclastogenesis, mononuclear osteoclast precursors form abundant TNTs in prior to cell-cell fusion. Here we introduce a protocol for detecting TNTs during osteoclastogenesis by use of live cell imaging utilizing a confocal laser microscopy. We also demonstrate a standard protocol for observation of TNTs by scanning electron microscope.

UR - http://www.scopus.com/inward/record.url?scp=84957056243&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84957056243&partnerID=8YFLogxK

U2 - 10.1007/978-1-4939-2703-6_14

DO - 10.1007/978-1-4939-2703-6_14

M3 - Chapter

AN - SCOPUS:84957056243

SN - 9781493927029

SP - 193

EP - 202

BT - Cell Fusion

PB - Springer New York

ER -