Membrane nanotube formation in osteoclastogenesis

Toshio Kukita, Akira Takahashi, Jing Qi Zhang, Akiko Kukita

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

Membrane tunneling nanotubes (TNTs) are unique intercellular structures, which enable rapid transport of various materials and rapid communication between cells present in a long distance. During osteoclastogenesis, mononuclear osteoclast precursors form abundant TNTs in prior to cell-cell fusion. Here we introduce a protocol for detecting TNTs during osteoclastogenesis by use of live cell imaging utilizing a confocal laser microscopy. We also demonstrate a standard protocol for observation of TNTs by scanning electron microscope.

Original languageEnglish
Title of host publicationCell Fusion
Subtitle of host publicationOverviews and Methods: Second Edition
PublisherSpringer New York
Pages193-202
Number of pages10
ISBN (Electronic)9781493927036
ISBN (Print)9781493927029
DOIs
Publication statusPublished - May 6 2015

All Science Journal Classification (ASJC) codes

  • Biochemistry, Genetics and Molecular Biology(all)

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