Memory phenotype CD8+ T cells in IL-15 transgenic mice are involved in early protection against a primary infection with Listeria monocytogenes

Toshiki Yajima, Hitoshi Nishimura, Ryotaro Ishimitsu, Kenichi Yamamura, Taketo Watase, Dirk H. Busch, Eric G. Pamer, Hiroyuki Kuwano, Yasunobu Yoshikai

    Research output: Contribution to journalArticle

    43 Citations (Scopus)

    Abstract

    We recently constructed IL-15 transgenic (Tg) mice using cDNA encoding a secretable isoform of the IL-15 precursor protein under the control of an MHC class I promoter. The IL-15 Tg mice exhibited resistance against a primary infection with Listeria monocytogenes. The numbers of memory CD8+ T cells were markedly increased in the IL-15 Tg mice following Listeria infection accompanied by sustained IL-15 production. The increased CD44+CD8+ T cells in the infected IL-15 Tg mice were not specialized to recognize Listeria-specific antigen but produced a large amount of IFN-γ in response to bystander stimulation exogenous IL-15 in combination with IL-12. Furthermore, Listeria-specific Th1 response by CD4+ T cells was significantly augmented in the IL-15 Tg mice compared with control mice following Listeria infection. In vivo depletion of the CD8+ T cells by anti-CD8 monoclonal antibody and adoptive transfer of the T cells from naive IL-15 Tg mice indicated that the CD8+ T cells functioned not only to eliminate bacteria at the early stage of infection but also to promote Th1 response to L. monocytogenes. Overexpression of IL-15 shed light on a novel role of memory CD8+ T cells in early protection and promotion of Th1 response against a primary infection with L. monocytogenes.

    Original languageEnglish
    Pages (from-to)757-766
    Number of pages10
    JournalEuropean Journal of Immunology
    Volume31
    Issue number3
    DOIs
    Publication statusPublished - Apr 5 2001

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    Interleukin-15
    Listeria monocytogenes
    Transgenic Mice
    T-Lymphocytes
    Phenotype
    Infection
    Listeriosis
    Listeria
    Protein Precursors
    Adoptive Transfer
    Interleukin-12
    Protein Isoforms
    Complementary DNA
    Monoclonal Antibodies
    Bacteria
    Antigens

    All Science Journal Classification (ASJC) codes

    • Immunology and Allergy
    • Immunology

    Cite this

    Memory phenotype CD8+ T cells in IL-15 transgenic mice are involved in early protection against a primary infection with Listeria monocytogenes. / Yajima, Toshiki; Nishimura, Hitoshi; Ishimitsu, Ryotaro; Yamamura, Kenichi; Watase, Taketo; Busch, Dirk H.; Pamer, Eric G.; Kuwano, Hiroyuki; Yoshikai, Yasunobu.

    In: European Journal of Immunology, Vol. 31, No. 3, 05.04.2001, p. 757-766.

    Research output: Contribution to journalArticle

    Yajima, Toshiki ; Nishimura, Hitoshi ; Ishimitsu, Ryotaro ; Yamamura, Kenichi ; Watase, Taketo ; Busch, Dirk H. ; Pamer, Eric G. ; Kuwano, Hiroyuki ; Yoshikai, Yasunobu. / Memory phenotype CD8+ T cells in IL-15 transgenic mice are involved in early protection against a primary infection with Listeria monocytogenes. In: European Journal of Immunology. 2001 ; Vol. 31, No. 3. pp. 757-766.
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