TY - JOUR
T1 - Meta-fermentation system with a mixed culture for the production of optically pure l-lactic acid can be reconstructed using the minimum isolates with a simplified pH control strategy
AU - Yue, Siyuan
AU - Mizoguchi, Takaharu
AU - Kohara, Toshiya
AU - Zhang, Min
AU - Watanabe, Kota
AU - Miyamoto, Hirokuni
AU - Tashiro, Yukihiro
AU - Sakai, Kenji
N1 - Funding Information:
This work was partly supported by MEXT KAKENHI Grant Number JP19H02875, by JSPS KAKENHI Grant Number 16K07670, and by the Lotte Research Promotion Grant. The authors would like to acknowledge the Center for Advanced Instrumental and Educational Supports, Faculty of Agriculture, Kyushu University, for their support with MiSeq. Siyuan Yue and Min Zhang gratefully acknowledge the Chinese government scholarship offered by the China Scholarship Council (201806460015, 201706460009).
Funding Information:
This work was partly supported by MEXT KAKENHI Grant Number JP19H02875, by JSPS KAKENHI Grant Number 16K07670, and by the Lotte Research Promotion Grant. The authors would like to acknowledge the Center for Advanced Instrumental and Educational Supports, Faculty of Agriculture, Kyushu University, for their support with MiSeq. Siyuan Yue and Min Zhang gratefully acknowledge the Chinese government scholarship offered by the China Scholarship Council (201806460015, 201706460009).
Publisher Copyright:
© 2021 Wiley-VCH GmbH
PY - 2021/11
Y1 - 2021/11
N2 - Meta-l-lactic acid fermentation from non-treated kitchen refuse was reconstructed using a combination of isolated bacterial strains under several pH control strategies. The meta-fermentation system was successfully reconstructed using a combination of Weizmannia coagulans MN-07, Caldibacillus thermoamylovorans OM55-6, and Caldibacillus hisashii N-11 strains. Additionally, a simplified constant pH control strategy was employed, which decreased fermentation time and increased production. The optimum pH (6.5) for the reconstructed meta-fermentation was favorable for the respective pure cultures of the three selected strains. The l-lactic acid production performance of the reconstructed meta-fermentation system was as follows: concentration, 24.5 g L−1; optical purity, 100%; productivity, 0.341 g L−1 h−1; yield, 1.06 g g−1. These results indicated that constant pH control was effective in the reconstructed meta-fermentation with the best performance of l-lactic acid production at pH optimal for the selected bacterial growth, while the switching from swing pH control would suppress the activities of unfavorable bacterial species in un-isolated meta-fermentation.
AB - Meta-l-lactic acid fermentation from non-treated kitchen refuse was reconstructed using a combination of isolated bacterial strains under several pH control strategies. The meta-fermentation system was successfully reconstructed using a combination of Weizmannia coagulans MN-07, Caldibacillus thermoamylovorans OM55-6, and Caldibacillus hisashii N-11 strains. Additionally, a simplified constant pH control strategy was employed, which decreased fermentation time and increased production. The optimum pH (6.5) for the reconstructed meta-fermentation was favorable for the respective pure cultures of the three selected strains. The l-lactic acid production performance of the reconstructed meta-fermentation system was as follows: concentration, 24.5 g L−1; optical purity, 100%; productivity, 0.341 g L−1 h−1; yield, 1.06 g g−1. These results indicated that constant pH control was effective in the reconstructed meta-fermentation with the best performance of l-lactic acid production at pH optimal for the selected bacterial growth, while the switching from swing pH control would suppress the activities of unfavorable bacterial species in un-isolated meta-fermentation.
UR - http://www.scopus.com/inward/record.url?scp=85115151271&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85115151271&partnerID=8YFLogxK
U2 - 10.1002/biot.202100277
DO - 10.1002/biot.202100277
M3 - Article
C2 - 34472222
AN - SCOPUS:85115151271
VL - 16
JO - Biotechnology Journal
JF - Biotechnology Journal
SN - 1860-6768
IS - 11
M1 - 2100277
ER -