TY - JOUR
T1 - Metabolic alteration of Methylococcus capsulatus str. Bath during a microbial gas-phase reaction
AU - Chen, Yan Yu
AU - Soma, Yuki
AU - Ishikawa, Masahito
AU - Takahashi, Masatomo
AU - Izumi, Yoshihiro
AU - Bamba, Takeshi
AU - Hori, Katsutoshi
N1 - Funding Information:
This work was supported by the Advanced Low Carbon Technology Research and Development Program (JPMJAL1402 and JPMJAL1607) of the Japan Science and Technology Agency, the project JPNP18016 of the New Energy and Industrial Technology Development Organization, Japan, KAKENHI grants JP18K14065 and JP17H06304 from the Japan Society for the Promotion of Science, and the Cooperative Research Project Program of the Medical Institute of Bioregulation, Kyushu University.
Publisher Copyright:
© 2021 Elsevier Ltd
PY - 2021/6
Y1 - 2021/6
N2 - This study demonstrates the metabolic alteration of Methylococcus capsulatus (Bath), a representative bacterium among methanotrophs, in microbial gas-phase reactions. For comparative metabolome analysis, a bioreactor was designed to be capable of supplying gaseous substrates and liquid nutrients continuously. Methane degradation by M. capsulatus (Bath) was more efficient in a gas-phase reaction operated in the bioreactor than in an aqueous phase reaction operated in a batch reactor. Metabolome analysis revealed remarkable alterations in the metabolism of cells in the gas-phase reaction; in particular, pyruvate, 2-ketoglutarate, some amino acids, xanthine, and hypoxanthine were accumulated, whereas 2,6-diaminopimelate was decreased. Based on the results of metabolome analysis, cells in the gas-phase reaction seemed to alter their metabolism to reduce the excess ATP and NADH generated upon increased availability of methane and oxygen. Our findings will facilitate the development of efficient processes for methane-based bioproduction with low energy consumption.
AB - This study demonstrates the metabolic alteration of Methylococcus capsulatus (Bath), a representative bacterium among methanotrophs, in microbial gas-phase reactions. For comparative metabolome analysis, a bioreactor was designed to be capable of supplying gaseous substrates and liquid nutrients continuously. Methane degradation by M. capsulatus (Bath) was more efficient in a gas-phase reaction operated in the bioreactor than in an aqueous phase reaction operated in a batch reactor. Metabolome analysis revealed remarkable alterations in the metabolism of cells in the gas-phase reaction; in particular, pyruvate, 2-ketoglutarate, some amino acids, xanthine, and hypoxanthine were accumulated, whereas 2,6-diaminopimelate was decreased. Based on the results of metabolome analysis, cells in the gas-phase reaction seemed to alter their metabolism to reduce the excess ATP and NADH generated upon increased availability of methane and oxygen. Our findings will facilitate the development of efficient processes for methane-based bioproduction with low energy consumption.
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U2 - 10.1016/j.biortech.2021.125002
DO - 10.1016/j.biortech.2021.125002
M3 - Article
C2 - 33770731
AN - SCOPUS:85103038444
SN - 0960-8524
VL - 330
JO - Agricultural Wastes
JF - Agricultural Wastes
M1 - 125002
ER -
