Metabolic analysis of mouse bone-marrow-derived dendritic cells using an extracellular flux analyzer

Kazuhito Gotoh, Yurie Takata, Yuya Nakashima, Soichi Mizuguchi, Keishi Komori, Dongchon Kang

Research output: Contribution to journalArticlepeer-review

Abstract

Dendritic cell (DC) maturation induced by Toll-like receptor (TLR) agonists requires the activation of downstream metabolic changes. Here, we provide a detailed protocol to measure glycolysis, mitochondrial respiration, and fatty acid oxidation in mouse bone-marrow-derived DCs with the Seahorse XF24 extracellular flux (XF) analyzer. XF analysis with the Seahorse bioanalyzer has become a standard method to measure bioenergetic functions in cells, and this protocol can be adapted to other immune cells. For complete information on using this protocol, please refer to Gotoh et al. (2018).

Original languageEnglish
Article number100401
JournalSTAR Protocols
Volume2
Issue number2
DOIs
Publication statusPublished - Jun 18 2021

All Science Journal Classification (ASJC) codes

  • Biochemistry, Genetics and Molecular Biology(all)
  • Neuroscience(all)
  • Immunology and Microbiology(all)

Fingerprint Dive into the research topics of 'Metabolic analysis of mouse bone-marrow-derived dendritic cells using an extracellular flux analyzer'. Together they form a unique fingerprint.

Cite this