TY - JOUR
T1 - Metabolic analysis of mouse bone-marrow-derived dendritic cells using an extracellular flux analyzer
AU - Gotoh, Kazuhito
AU - Takata, Yurie
AU - Nakashima, Yuya
AU - Mizuguchi, Soichi
AU - Komori, Keishi
AU - Kang, Dongchon
N1 - Funding Information:
This work was supported by JSPS KAKENHI grant numbers JP18K11077 and JP16K19196 to K.G. and JP20H00530 and JP17H01550 to D.K. This work was also supported by a grant from the Takeda Science Foundation , The Shin-Nihon Foundation of Advanced Medical Research , and Charitable Trust Laboratory Medicine Research Foundation of Japan (to K.G.). We would like to acknowledge all of our colleagues in Dr. Kang's and Dr. Fukui's laboratory for their support throughout this project. We thank Edanz Group ( https://en-author-services.edanz.com/ac ) for editing a draft of this manuscript.
Publisher Copyright:
© 2021 The Author(s)
PY - 2021/6/18
Y1 - 2021/6/18
N2 - Dendritic cell (DC) maturation induced by Toll-like receptor (TLR) agonists requires the activation of downstream metabolic changes. Here, we provide a detailed protocol to measure glycolysis, mitochondrial respiration, and fatty acid oxidation in mouse bone-marrow-derived DCs with the Seahorse XF24 extracellular flux (XF) analyzer. XF analysis with the Seahorse bioanalyzer has become a standard method to measure bioenergetic functions in cells, and this protocol can be adapted to other immune cells. For complete information on using this protocol, please refer to Gotoh et al. (2018).
AB - Dendritic cell (DC) maturation induced by Toll-like receptor (TLR) agonists requires the activation of downstream metabolic changes. Here, we provide a detailed protocol to measure glycolysis, mitochondrial respiration, and fatty acid oxidation in mouse bone-marrow-derived DCs with the Seahorse XF24 extracellular flux (XF) analyzer. XF analysis with the Seahorse bioanalyzer has become a standard method to measure bioenergetic functions in cells, and this protocol can be adapted to other immune cells. For complete information on using this protocol, please refer to Gotoh et al. (2018).
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U2 - 10.1016/j.xpro.2021.100401
DO - 10.1016/j.xpro.2021.100401
M3 - Article
AN - SCOPUS:85103321666
VL - 2
JO - STAR Protocols
JF - STAR Protocols
SN - 2666-1667
IS - 2
M1 - 100401
ER -