Metabolic analysis of mouse bone-marrow-derived dendritic cells using an extracellular flux analyzer

Kazuhito Gotou; Yurie Takata; Yuya Nakashima; Soichi Mizuguchi; Keishi Komori; Dongchon Kang

doi:10.1016/j.xpro.2021.100401

Metabolic analysis of mouse bone-marrow-derived dendritic cells using an extracellular flux analyzer

Kazuhito Gotou, Yurie Takata, Yuya Nakashima, Soichi Mizuguchi, Keishi Komori, Dongchon Kang

Research output: Contribution to journal › Article › peer-review

5 Citations (Scopus)

Abstract

Dendritic cell (DC) maturation induced by Toll-like receptor (TLR) agonists requires the activation of downstream metabolic changes. Here, we provide a detailed protocol to measure glycolysis, mitochondrial respiration, and fatty acid oxidation in mouse bone-marrow-derived DCs with the Seahorse XF24 extracellular flux (XF) analyzer. XF analysis with the Seahorse bioanalyzer has become a standard method to measure bioenergetic functions in cells, and this protocol can be adapted to other immune cells. For complete information on using this protocol, please refer to Gotoh et al. (2018).

Original language	English
Article number	100401
Journal	STAR Protocols
Volume	2
Issue number	2
DOIs	https://doi.org/10.1016/j.xpro.2021.100401
Publication status	Published - Jun 18 2021

All Science Journal Classification (ASJC) codes

Biochemistry, Genetics and Molecular Biology(all)
Neuroscience(all)
Immunology and Microbiology(all)

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10.1016/j.xpro.2021.100401

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title = "Metabolic analysis of mouse bone-marrow-derived dendritic cells using an extracellular flux analyzer",

abstract = "Dendritic cell (DC) maturation induced by Toll-like receptor (TLR) agonists requires the activation of downstream metabolic changes. Here, we provide a detailed protocol to measure glycolysis, mitochondrial respiration, and fatty acid oxidation in mouse bone-marrow-derived DCs with the Seahorse XF24 extracellular flux (XF) analyzer. XF analysis with the Seahorse bioanalyzer has become a standard method to measure bioenergetic functions in cells, and this protocol can be adapted to other immune cells. For complete information on using this protocol, please refer to Gotoh et al. (2018).",

author = "Kazuhito Gotou and Yurie Takata and Yuya Nakashima and Soichi Mizuguchi and Keishi Komori and Dongchon Kang",

note = "Funding Information: This work was supported by JSPS KAKENHI grant numbers JP18K11077 and JP16K19196 to K.G. and JP20H00530 and JP17H01550 to D.K. This work was also supported by a grant from the Takeda Science Foundation , The Shin-Nihon Foundation of Advanced Medical Research , and Charitable Trust Laboratory Medicine Research Foundation of Japan (to K.G.). We would like to acknowledge all of our colleagues in Dr. Kang's and Dr. Fukui's laboratory for their support throughout this project. We thank Edanz Group ( https://en-author-services.edanz.com/ac ) for editing a draft of this manuscript. Publisher Copyright: {\textcopyright} 2021 The Author(s)",

year = "2021",

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doi = "10.1016/j.xpro.2021.100401",

language = "English",

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T1 - Metabolic analysis of mouse bone-marrow-derived dendritic cells using an extracellular flux analyzer

AU - Gotou, Kazuhito

AU - Takata, Yurie

AU - Nakashima, Yuya

AU - Mizuguchi, Soichi

AU - Komori, Keishi

AU - Kang, Dongchon

N1 - Funding Information: This work was supported by JSPS KAKENHI grant numbers JP18K11077 and JP16K19196 to K.G. and JP20H00530 and JP17H01550 to D.K. This work was also supported by a grant from the Takeda Science Foundation , The Shin-Nihon Foundation of Advanced Medical Research , and Charitable Trust Laboratory Medicine Research Foundation of Japan (to K.G.). We would like to acknowledge all of our colleagues in Dr. Kang's and Dr. Fukui's laboratory for their support throughout this project. We thank Edanz Group ( https://en-author-services.edanz.com/ac ) for editing a draft of this manuscript. Publisher Copyright: © 2021 The Author(s)

PY - 2021/6/18

Y1 - 2021/6/18

N2 - Dendritic cell (DC) maturation induced by Toll-like receptor (TLR) agonists requires the activation of downstream metabolic changes. Here, we provide a detailed protocol to measure glycolysis, mitochondrial respiration, and fatty acid oxidation in mouse bone-marrow-derived DCs with the Seahorse XF24 extracellular flux (XF) analyzer. XF analysis with the Seahorse bioanalyzer has become a standard method to measure bioenergetic functions in cells, and this protocol can be adapted to other immune cells. For complete information on using this protocol, please refer to Gotoh et al. (2018).

AB - Dendritic cell (DC) maturation induced by Toll-like receptor (TLR) agonists requires the activation of downstream metabolic changes. Here, we provide a detailed protocol to measure glycolysis, mitochondrial respiration, and fatty acid oxidation in mouse bone-marrow-derived DCs with the Seahorse XF24 extracellular flux (XF) analyzer. XF analysis with the Seahorse bioanalyzer has become a standard method to measure bioenergetic functions in cells, and this protocol can be adapted to other immune cells. For complete information on using this protocol, please refer to Gotoh et al. (2018).

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Metabolic analysis of mouse bone-marrow-derived dendritic cells using an extracellular flux analyzer

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