Metabolic function of glycogen phosphorylase and trehalose phosphorylase in fruit-body formation of Flammulina velutipes

Yukata Kitamoto, Atsushi Kobayashi, Nobuhiro Mori, Shoji Ohga

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Glycogen phosphorylase in the vegetative mycelium of Flammulina velutipes converts glycogen to β-glucose 1-phosphate (G1P) in the colony during fruit-body development. Glycogen may contribute to the synthesis of trehalose as the starting material in the vegetative mycelium during the fruiting process of the colony, and the trehalose produced is translocated into the fruit-bodies as the main carbohydrate substrate for their development. Trehalose phosphorylase activity in the vegetative mycelium was at a relatively high level until fruit-body initiation, suggesting the turnover of this disaccharide during the vegetative stage of the colony development. Trehalose phosphorylase activity in the stipes showed a peak level at the early phase of fruit-body development, suggesting the continuing phosphorolysis of trehalose by this enzyme. The stipes also showed a high specific activity of phosphoglucomutase at a sufficient level to facilitate the conversion of G1P to β-glucose 6-phosphate (G6P). In the pilei a large amount of G1P remained until the growth of the fruit-bodies ceased. Trehalase activities in the stipes and pilei were at a very low level, and this enzyme may not contribute to the catabolism of trehalose in the fruit-body development.

Original languageEnglish
Pages (from-to)143-147
Number of pages5
JournalMycoscience
Volume42
Issue number2
DOIs
Publication statusPublished - Apr 15 2001

Fingerprint

Flammulina velutipes
phosphorylase
trehalose
fruiting bodies
glycogen
fruit
glucose 1-phosphate
glucose
phosphate
mycelium
enzyme
disaccharide
trehalase
phosphoglucomutase
glucose 6-phosphate
catabolism
disaccharides
fruiting
enzymes
vegetative growth

All Science Journal Classification (ASJC) codes

  • Ecology, Evolution, Behavior and Systematics

Cite this

Metabolic function of glycogen phosphorylase and trehalose phosphorylase in fruit-body formation of Flammulina velutipes. / Kitamoto, Yukata; Kobayashi, Atsushi; Mori, Nobuhiro; Ohga, Shoji.

In: Mycoscience, Vol. 42, No. 2, 15.04.2001, p. 143-147.

Research output: Contribution to journalArticle

Kitamoto, Yukata ; Kobayashi, Atsushi ; Mori, Nobuhiro ; Ohga, Shoji. / Metabolic function of glycogen phosphorylase and trehalose phosphorylase in fruit-body formation of Flammulina velutipes. In: Mycoscience. 2001 ; Vol. 42, No. 2. pp. 143-147.
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